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Chapter 12,562

Site-directed mutagenesis of active site residues of phosphite dehydrogenase

Woodyer, R.; Wheatley, J.L.; Relyea, H.A.; Rimkus, S.; van der Donk, W.A.

Biochemistry 44(12): 4765-4774

2005

ISSN/ISBN: 0006-2960
PMID: 15779903
DOI: 10.1021/bi047868c
Accession: 012561974
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Phosphite dehydrogenase (PTDH) catalyzes the unusual oxidation of phosphite to phosphate with the concomitant reduction of NAD(+) to NADH. PTDH shares significant amino acid sequence similarity with D-hydroxy acid dehydrogenases (DHs), including strongly conserved catalytic residues His292, Glu266, and Arg237. Site-directed mutagenesis studies corroborate the essential role of His292 as all Mutants of this residue were completely inactive.

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