Section 13
Chapter 12,563

Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase

Parikh, M.R.; Matsumura, I.

Journal of Molecular Biology 352(3): 621-628


ISSN/ISBN: 0022-2836
PMID: 16095606
DOI: 10.1016/j.jmb.2005.07.020
Accession: 012562032

Protein engineers use a variety of mutagenic strategies to adapt enzymes to novel substrates. Directed evolution techniques (random mutagenesis and high-throughput screening) offer a systematic approach to the management of protein complexity. This sub-discipline was galvanized by the invention of DNA shuffling, a procedure that randomly recombines point mutations in vitro.

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