Solid-state fermentation of lignocellulosic substrates for cellulase production by Trichoderma reesei NRRL 11460

Singhania, R.-Rani; Sukumaran, R., K.; Pillai, A.; Szakacs, G.; Pandey, A.

Indian Journal of Biotechnology 5(3): 332-336


ISSN/ISBN: 0972-5849
Accession: 012567878

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Cellulase production studies were carried out using the fungal culture Trichoderma reesei NRRL 11460 using four different lignocellulosic residues (both raw and pre-treated) by solid-state fermentation. The effect of basic fermentation parameters on enzyme production was studied. Maximal cellulase production obtained was 154.58 U/gds when pre-treated sugarcane bagasse (PSCB) was used as substrate. The optimal conditions for cellulase production using PSCB were found to be initial moisture content -66%, initial medium pH-7.0, incubation temperature -28 degrees C, NH4NO3 at 0.075 M, and 0.005 M cellobiose. The optimal incubation time for production was 72 h. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF.