EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Spinal circuits formation: a study of developmentally regulated markers in organotypic cultures of embryonic mouse spinal cord


Spinal circuits formation: a study of developmentally regulated markers in organotypic cultures of embryonic mouse spinal cord



Neuroscience 122(2): 391-405



ISSN/ISBN: 0306-4522

PMID: 14614905

DOI: 10.1016/j.neuroscience.2003.07.006

In this study, we have addressed the issue of neural circuit formation using the mouse spinal cord as a model system. Our primary objective was to assess the suitability of organotypic cultures from embryonic mouse spinal cord to investigate, during critical periods of spinal network formation, the role of the local spinal cellular environment in promoting circuit development and refinement. These cultures offer the great advantage over other in vitro systems, of preserving the basic cytoarchitecture and the dorsal-ventral orientation of the spinal segment from which they are derived [Eur J Neurosci 14 (2001) 903; Eur J Neurosci 16 (2002) 2123]. Long-term embryonic spinal cultures were developed and analyzed at sequential times in vitro, namely after 1, 2, and 3 weeks. Spatial and temporal regulation of neuronal and non-neuronal markers was investigated by immunocytochemical and Western blotting analysis using antibodies against: a) the non-phosphorylated epitope of neurofilament H (SMI32 antibody); b) the enzyme choline acetyltransferase, to localize motoneurons and cholinergic interneurons; c) the enzyme glutamic acid decarboxylase 67, to identify GABAergic interneurons; d) human eag-related gene (HERG) K(+) channels, which appear to be involved in early stages of neuronal and muscle development; e) glial fibrillary acidic protein, to identify mature astrocytes; f) myelin basic protein, to identify the onset of myelination by oligodendrocytes. To examine the development of muscle acetylcholine receptors clusters in vitro, we incubated live cultures with tetramethylrhodamine isothiocyanate-labeled alpha-bungarotoxin, and we subsequently immunostained them with SMI32 or with anti-myosin antibodies. Our results indicate that the developmental pattern of expression of these markers in organotypic cultures shows close similarities to the one observed in vivo. Therefore, spinal organotypic cultures provide a useful in vitro model system to study several aspects of neurogenesis, gliogenesis, muscle innervation, and synaptogenesis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012576985

Download citation: RISBibTeXText

Related references

Basal lamina formation by astrocytes in organotypic cultures of mouse spinal cord tissue. Journal of Neuropathology and Experimental Neurology 44(3): 295-303, 1985

Network bursting by organotypic spinal slice cultures in the presence of bicuculline and/or strychnine is developmentally regulated. European Journal of Neuroscience 10(9): 2871-2879, 1998

An Organotypic Spinal Cord - Dorsal Root Ganglion - Skeletal Muscle Coculture of Embryonic Rat. II. Functional Evidence for the Formation of Spinal Reflex Arcs in Vitro. European Journal of Neuroscience 3(11): 1054-1068, 1991

Characterization of novel developmentally regulated proteins in embryonic rat spinal cord. Society for Neuroscience Abstracts 17(1-2): 540, 1991

Intrinsic activity and positive feedback in motor circuits in organotypic spinal cord slice cultures. European Journal of Neuroscience 30(8): 1487-1497, 2009

Development of neurotensin-immunoreactive nerve cells in organotypic cultures of embryonic rat spinal cord. Neuroscience Letters 19(2): 185-190, 1980

Central projections of sensory neurons in organotypic cultures of embryonic chick spinal cord. Society for Neuroscience Abstracts 18(1-2): 219, 1992

Developmentally regulated markers in the postnatal cervical spinal cord of the opossum Monodelphis domestica. Brain Research. Developmental Brain Research 103(1): 47-57, 1997

Taxol effects on glia in organotypic mouse spinal cord-DRG cultures. Cell Biology International Reports 9(6): 539-546, 1985

Lipid and enzymatic determinations of organotypic cultures of mouse spinal cord. Journal of Neurochemistry 41(Suppl): S153, 1983

Gliogenesis in organotypic tissue culture of the spinal cord of the embryonic mouse. II. Autoradiographic studies. Journal of Neurocytology 15(3): 291-302, 1986

Gliogenesis in organotypic tissue culture of the spinal cord of the embryonic mouse. I. Immunocytochemical and ultrastructural studies. Journal of Neurocytology 15(3): 273-290, 1986

Development of tolerance to opiates and opioid peptides in organotypic cultures of mouse spinal cord. Life Sciences 25(21): 1797-1802, 1979

Regional differences in astrocyte organization in organotypic cultures of mouse spinal cord tissue. Journal of Neuropathology and Experimental Neurology 44(3): 343, 1985

Testosterone increases cell numbers in organotypic cultures of fetal mouse spinal cord. Society for Neuroscience Abstracts 11(2): 898, 1985