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Studies on molecular cytogenetics of wheat-Psathyrostachys huashanica germplasm with resistance to wheat take-all fungus

Studies on molecular cytogenetics of wheat-Psathyrostachys huashanica germplasm with resistance to wheat take-all fungus

Xibei Zhiwu Xuebao 23(11): 1905-1909

The molecular cytogenetics and disease resistance of addition line H2O and substitution line H1 from wheat-P. huashanica (2n=14, NN) were studied. The results showed that the somatic chromosome numbers were 42 to 44 from H2O and 40 to 42 from H1, and the cells rates of 2n=44 and 2n=42 were 58.33% and 90% respectively. The chromosome configurations were 21.55 II+0.90 I from H2O and 21 II+0. 52 I from H1, the cells of 22 II and 21 II were accounted for 61.56% and 86.18% respectively and after test crossed with CS, the percentages of 21 II+1 I and 20 II+2 I were 70.14% and 88.59% respectively in PMC at MI. The in situ hybridization using P. huashanica genomic DNA as probe showed that H2O and H1 possessed 2 chromosomes of P. huashanica and their chromosome constitutions could be expressed as:2n=44=42W+2N and 2n=42=40W+2N respectively. H2O behaved close to high resistance and H1 showed middle resistance to wheat take-all fungus.

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Accession: 012593483

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