Syntheses enzymatiques de neoglucoconjugues catalysees par l'alpha-glucosidase purifiee de la blatte Periplaneta americana (Linnaeus)
Kouame, L.-Patrice; Due, E.-Ahipo; Niamke, S.-Lamine; Kouame, F.-Akissi; Kamenan, A.
Biotechnologie Agronomie Societe et Environnement 9(1): 35-42
ISSN/ISBN: 1370-6233 Accession: 012609150
Cockroach Periplaneta americana (Linnaeus) contains in his digestive tract an acid (pH 5,0) and mesophile (50 degrees C) alpha-glucosidase. This enzyme, purified to homogeneity, hydrolyses highly maltose, sucrose and p-nitrophenyl-alpha-D-glucopyranoside. The ability of alpha-glucosidase from cockroach purified to homogeneity to catalyse transglucosylation reactions was tested using maltose and saccharose as glucosyl donors and 2-phenylethanol and phenol as acceptors. The experimental conditions were optimized in relation to the time course of the reaction, pH and concentrations of glucosyl donors and acceptors.