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Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli

Thompson, J.; Dahlberg, A.E.

Nucleic Acids Research 32(19): 5954-5961

2004


ISSN/ISBN: 0305-1048
PMID: 15534366
DOI: 10.1093/nar/gkh925
Accession: 012625090

Ribosomes from the extreme thermophile Thermus thermophilus are capable of translation in a coupled transcription-translation system derived from Escherichia coli. At 45degreeC, T.thermophilus ribosomes translate at apprx25-30% of the maximal rate of E.coli ribosomes, and synthesize full-length protein. T.thermophilus and E.coli subunits can be combined to effect translation, with the spectrum of proteins produced depending upon the source of the 30S subunit.

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