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Testing the conservation of the translational machinery over evolution in diverse environments: assaying Thermus thermophilus ribosomes and initiation factors in a coupled transcription-translation system from Escherichia coli

Thompson, J.; Dahlberg, A.E.

Nucleic Acids Research 32(19): 5954-5961

2004

ISSN/ISBN: 0305-1048
PMID: 15534366
DOI: 10.1093/nar/gkh925
Accession: 012625090
Ribosomes from the extreme thermophile Thermus thermophilus are capable of translation in a coupled transcription-translation system derived from Escherichia coli. At 45degreeC, T.thermophilus ribosomes translate at apprx25-30% of the maximal rate of E.coli ribosomes, and synthesize full-length protein. T.thermophilus and E.coli subunits can be combined to effect translation, with the spectrum of proteins produced depending upon the source of the 30S subunit.

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Related References

Wolfrum, A.; Brock, S.; Mac, T.; Grillenbeck, N. 2003: Expression in E. coli and purification of Thermus thermophilus translation initiation factors IF1 and IF3 Protein Expression and Purification 29(1): 15-23
Kapralou, S.; Fabbretti, A.; Garulli, C.; Spurio, R.; Gualerzi, C.O.; Dahlberg, A.E.; Pon, C.L. 2008: Translation initiation factor IF1 of Bacillus stearothermophilus and Thermus thermophilus substitute for Escherichia coli IF1 in vivo and in vitro without a direct IF1-IF2 interaction Molecular Microbiology 70(6): 1368-1377
Iwasaki, K.; Sabol, S.; Wahba, A.J.; Ochoa, S. 1968: Translation of the genetic message. VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes Archives of Biochemistry and Biophysics 125(2): 542-547
Perederina, A.A.; Vassylyeva, M.N.; Berezin, I.A.; Svetlov, V.; Artsimovitch, I.; Vassylyev, D.G. 2006: Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus Acta Crystallographica. Section F Structural Biology and Crystallization Communications 62(Part 1): 44-46
Karginov, A.V.; Karginova, O.A.; Spiridonova, V.A.; Kopylov, A.M. 1995: In vivo assembly of plasmid-expressed ribosomal protein S7 of Thermus thermophilus into Escherichia coli ribosomes and conditions of its overexpression Febs Letters 369(2-3): 158-160
Nakamura, A.; Takakura, Y.; Kobayashi, H.; Hoshino, T. 2005: In vivo directed evolution for thermostabilization of Escherichia coli hygromycin B phosphotransferase and the use of the gene as a selection marker in the host-vector system of Thermus thermophilus Journal of Bioscience and Bioengineering 100(2): 158-163
Babkina, G.T.; Karpova, G.G.; Matasova, N.B.; Berzin', V.M.; Gren, E.Ia. 1985: Study of the mRNA-binding region of ribosomes at different steps of translation. II. Affinity modification of Escherichia coli ribosomes by benzylidene derivative of AUGU6 in the 70S initiation complex Molekuliarnaia Biologiia 19(4): 1079-1085
Köhrer, C.; Mayer, C.; Gröbner, P.; Piendl, W. 1996: Use of T7 RNA polymerase in an optimized Escherichia coli coupled in vitro transcription-translation system. Application in regulatory studies and expression of long transcription units European Journal of Biochemistry 236(1): 234-239
Papadopoulos, G.; Grudinin, S.; Kalpaxis, D.L.; Choli-Papadopoulou, T. 2006: Changes in the level of poly(Phe) synthesis in Escherichia coli ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus can be explained by structural changes in the peptidyltransferase center: a molecular dynamics simulation analysis European Biophysics Journal: Ebj 35(8): 675-683
Blank, J.; Grillenbeck, N.W.; Kreutzer, R.; Sprinzl, M. 1995: Overexpression and purification of Thermus thermophilus elongation factors G, Tu, and Ts from Escherichia coli Protein Expression and Purification 6(5): 637-645
Celano, B.; Pawlik, R.T.; Gualerzi, C.O. 1988: Interaction of Escherichia coli translation-initiation factor IF-1 with ribosomes European Journal of Biochemistry 178(2): 351-355
Venkstern, T.V.; Graifer, D.M.; Karpova, G.G.; Morozov, I.A. 1990: Studies of the interaction of a transfer rna phe derivative bearing an arylazide group on its g 24 residue with escherichia coli ribosomes and transfer rna adenine 1 methyltransferase from thermus thermophilus Biopolimery i Kletka 6(4): 59-65
Calogero, R.A.; Pon, C.L.; Canonaco, M.A.; Gualerzi, C.O. 1988: Selection of the mRNA translation initiation region by Escherichia coli ribosomes Proceedings of the National Academy of Sciences of the United States of America 85(17): 6427-6431
Jiang, X.; Ookubo, Y.; Fujii, I.; Nakano, H.; Yamane, T. 2002: Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system FEBS Letters 514(2-3): 290-294
Moorman, A.F.; Lamie, F.; Grivell, L.A. 1976: A coupled transcription--translation system derived from Escherichia coli: the use of immobilized deoxyribonuclease to eliminate endogenous DNA Febs Letters 72(1): 67-72
Zoldák, G.; Redecke, L.; Svergun, D.I.; Konarev, P.V.; Voertler, C.S.; Dobbek, H.; Sedlák, E.; Sprinzl, M. 2007: Release factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies Nucleic Acids Research 35(4): 1343-1353
Gavrilova, L.P.; Smolyaninov, V.V. 1971: Study of the mechanism of translocation in ribosomes. 1. Polyphenylalanine synthesis in Escherichia coli ribosomes without participation of guanosine-5'-triphosphate and protein translation factors Molecular Biology 5(6): 710-717
Yamagishi, K.; Oshima, T.; Masuda, Y.; Ara, T.; Kanaya, S.; Mori, H. 2002: Conservation of translation initiation sites based on dinucleotide frequency and codon usage in Escherichia coli K-12 (W3110): non-random distribution of A/T-rich sequences immediately upstream of the translation initiation codon Dna Research: An International Journal for Rapid Publication of Reports on Genes and Genomes 9(1): 19-24
Sapiro, K.T.S.; Ilan, J. 1981: Transcription translation linked system effect of the components of the translational machinery on the rate and quality of transcription by isolated cockerel liver nuclei Journal of Cell Biology 91(2 Part 2): 361A
Vornlocher, H.P.; Scheible, W.R.; Faulhammer, H.G.; Sprinzl, M. 1997: Identification and purification of translation initiation factor 2 (IF2) from Thermus thermophilus European Journal of Biochemistry 243(1-2): 66-71