The application of infrequent-restriction-site PCR as a typing method of Staphylococcus aureus
Dai-Wenxia; Shen-Xu-Zhuang; Zhou-Hong; Gao-Wei; Zhang-Gui-Rong; Yu-Sang-Jie; Liu-Yan-Jie; Qi-Hong-Yan; Yang-Yong-Hong
Zhonghua Weishengwuxue He Mianyixue Zazhi 25(6): 514-518
Objective To explore the value of infrequent-restriction-site PCR (IRS-PCR) as a typing method of Staphylococcus aureus(Sa). Methods Typing all strains by pulse-field gel electrophoresis(PFGE) and IRS-PCR. Discriminatory index (ID) was measured according to the typing situation of 49 community acquired (CA) strains. Repeating the typing process of 30 strains to examine reproducibility. Typing ability, discriminatory ability, reproducibility, accordance ratio and performance of two genotyping methods were compared. Results IRS-PCR as a genotyping method for Sa was established. 70 strains were all typed by two methods, and typing ability of two methods were both 100%.