+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The fibrinogen receptor FbsA promotes adherence of Streptococcus agalactiae to human epithelial cells

The fibrinogen receptor FbsA promotes adherence of Streptococcus agalactiae to human epithelial cells

Infection and Immunity 72(11): 6197-6205

Streptococcus agalactiae is a major cause of bacterial pneumonia, sepsis, and meningitis in human neonates. During the course of infection, S. agalactiae adheres to a variety of epithelial cells but the underlying mechanisms are only poorly understood. The present report demonstrates the importance of the fibrinogen receptor FbsA for the streptococcal adherence and invasion of epithelial cells. Deletion of the fbsA gene in various S. agalactiae strains substantially reduced their binding of soluble fibrinogen and their adherence to and invasion of epithelial cells, indicating a role of FbsA in these different processes. The adherence and invasiveness of an fbsA deletion mutant were partially restored by reintroducing the fbsA gene on an expression vector. Heterologous expression of fbsA in Lactococcus lactis enabled this bacterium to adhere to but not to invade epithelial cells, suggesting that FbsA is a streptococcal adhesin. Flow cytometry experiments revealed a dose-dependent binding of FbsA to the surface of epithelial cells. Furthermore, tissue culture experiments exhibited an intimate contact of FbsA-coated latex beads with the surfaces of human epithelial cells. Finally, host cell adherence and invasion were significantly blocked in competition experiments with either purified FbsA protein or a monoclonal antibody directed against the fibrinogen-binding epitope of FbsA. Taken together, our studies demonstrate that FbsA promotes the adherence of S. agalactiae to epithelial cells but that FbsA does not mediate the bacterial invasion into host cells. Our results also indicate that fibrinogen-binding epitopes within FbsA are involved in the adherence of S. agalactiae to epithelial cells.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 012654637

Download citation: RISBibTeXText

PMID: 15501744

DOI: 10.1128/iai.72.11.6197-6205.2004

Related references

The fibrinogen-receptor FbsA mediates binding of Streptococcus agalactiae to human epithelial cells. Abstracts of the General Meeting of the American Society for Microbiology 103: B-097, 2003

Adherence to and invasion of human brain microvascular endothelial cells are promoted by fibrinogen-binding protein FbsA of Streptococcus agalactiae. Infection and Immunity 73(7): 4404-4409, 2005

Expression, purification and structural analysis of a fibrinogen receptor FbsA from Streptococcus agalactiae. Protein Journal 30(3): 159-166, 2011

The surface protein Srr-1 of Streptococcus agalactiae binds human keratin 4 and promotes adherence to epithelial HEp-2 cells. Infection and Immunity 75(11): 5405-5414, 2007

The novel fibrinogen-binding protein FbsB promotes Streptococcus agalactiae invasion into epithelial cells. Infection and Immunity 72(6): 3495-3504, 2004

Evaluation of the ability of Streptococcus agalactiae strains isolated from genital and neonatal specimens to bind to human fibrinogen and correlation with characteristics of the fbsA and fbsB genes. Infection and Immunity 75(3): 1310-1317, 2007

FbsA, a fibrinogen-binding protein from Streptococcus agalactiae, mediates platelet aggregation. Blood 105(3): 1052-1059, 2005

FbsA, a fibrinogen-binding protein from Streptococcus agalactiae, mediates platelet aggregation. Blood 105(3): 1052-1059, 2005

Multiple interactions of FbsA, a surface protein from Streptococcus agalactiae, with fibrinogen: affinity, stoichiometry, and structural characterization. Biochemistry 45(42): 12840-12852, 2006

The transcriptional regulator Rgg3 controls fibrinogen-binding of Streptococcus agalactiae and bacterial adherence to epithelial. 2007

Dual role for pilus in adherence to epithelial cells and biofilm formation in Streptococcus agalactiae. Plos Pathogens 5(5): E1000422, 2009

Role of pilus proteins in adherence and invasion of Streptococcus agalactiae to the lung and cervical epithelial cells. Journal of Biological Chemistry 288(6): 4023-4034, 2013

The role of streptococcus agalactiae selective adherence to epithelial cells of the cow mammary gland in the onset of mastitis. Veterinarski Glasnik 41(11-12): 1034-1037, 1987

Immunogenic properties of Streptococcus agalactiae FbsA fragments. Plos one 8(9): E75266, 2013

Effect of two probiotic strains of Lactobacillus on in vitro adherence of Listeria monocytogenes, Streptococcus agalactiae, and Staphylococcus aureus to vaginal epithelial cells. Current Microbiology 68(6): 679-684, 2014