The influence of different light intensity and spectral composition on pigment apparatus and functional characteristics of pea chloroplasts (Pisum sativum L.) Delta Lambda

Topchiy, N.M.; Syvash, O.O.; Fomishina, R.M.

Ukrayins'kyi Botanichnyi Zhurnal 61(5): 91-98

2004


Accession: 012661026

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Abstract
The aim of this work was to study the effect of various light intensity and spectral composition of illumination on pigment content and functional characteristics of pea chloroplasts. Pea plants (Pisum sativum L.) were grown in Knoppa medium for 14 days under two intensities: high (310 mmol . m(-2) . s(-1)) and low (60 mmol . m(-1) . s(-1)). The red LEDs (lambda(max) = 660 nm, Delta lambda = 20 nm, 15 mmol . m(-2) . s(-1)) were added to white light both intensities. The data confirmed that Chl a, b and carotenoids content in leaves are dependent on the intensity and spectral composition of light. It was shown that plants grown under red-enriched light had higher content of pigments compated to those grown in white light, but Chl a/b and Chls/Car ratios were practically invariable. The data shown that the rate of uncoupled electron transport was higher in thylakoids isolated from pea leaves grown at additional red illumination. The amount of chlorophyll a in lightharvesting coplex (IHC) and pigment-protein complexes of photosystems I and II was also higher in plants grown under red-enriched light. Hence, these data show that addition of red LEDs light resulted in some changes in composition of the photosynthetic apparatus.