EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Thermodynamic stability and formation of aggregates of human immunoglobulin G characterised by differential scanning calorimetry and dynamic light scattering


Thermodynamic stability and formation of aggregates of human immunoglobulin G characterised by differential scanning calorimetry and dynamic light scattering



Journal of Biochemical and Biophysical Methods 66(1-3): 73-86



ISSN/ISBN: 0165-022X

PMID: 16458360

DOI: 10.1016/j.jbbm.2005.12.003

The final process step of polyclonal human immunoglobulin G is formulation with agents such as sugars, polyols, amino acid and salts. Often the most stable formulations were empirically identified. Physicochemical methods, such as differential scanning calorimetry and dynamic light scattering, provide a deeper insight on the biophysical properties of such a protein solution. The combination of these methods proved to be sensitive enough to detect fine differences in the properties relevant for the development of stable protein solutions. The influence of additives, such as maltose and glycine in combination with water or low concentrations of salts, on human immunoglobulin preparations was analysed. Differential scanning calorimetry illustrated that 0.2 M glycine had better stabilising effects compared to 10% maltose. Dynamic light scattering and differential scanning calorimetry revealed that solutions preventing aggregation were not optimal in terms of thermodynamic stability. Aggregation was minimised with increasing ionic strength, shown by dynamic light scattering, whereas thermodynamic stability for heat sensitive parts of human immunoglobulin G, analysed with differential scanning calorimetry, was decreased.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012690445

Download citation: RISBibTeXText

Related references

Probing the combined effect of flunitrazepam and lidocaine on the stability and organization of bilayer lipid membranes. a differential scanning calorimetry and dynamic light scattering study. Cell Biochemistry and Biophysics 66(3): 461-475, 2013

Studying the curing of epoxyamine mixtures via dynamic light scattering and differential scanning calorimetry. Russian Journal of Physical Chemistry A, Focus on Chemistry 87(1): 145-148, 2013

Analysis of IgG kinetic stability by differential scanning calorimetry, probe fluorescence and light scattering. Protein Science: a Publication of the Protein Society 26(11): 2229-2239, 2017

Thermal stability of black carbon characterised by oxidative differential scanning calorimetry. Organic Geochemistry 38(1): 112-127, 2007

Properties of mixed DOTAP-DPPC bilayer membranes as reported by differential scanning calorimetry and dynamic light scattering measurements. Journal of Physical Chemistry. B 111(33): 10032-10039, 2007

Analysis of aggregates of human immunoglobulin G using size-exclusion chromatography, static and dynamic light scattering. Journal of Chromatography. a 1009(1-2): 89-96, 2003

Kinetics of symmetric and asymmetric phospholipid transfer between small sonicated vesicles studied by high sensitivity differential scanning calorimetry nmr electron microscopy and dynamic light scattering. Biochemistry 27(16): 6078-6085, 1988

Kinetics of symmetric and asymmetric phospholipid transfer between small sonicated vesicles studied by high-sensitivity differential scanning calorimetry, NMR, electron microscopy, and dynamic light scattering. Biochemistry (Easton) 27(16): 6078-6085, 1988

The thermodynamic stability of amyloid fibrils studied by differential scanning calorimetry. Journal of Physical Chemistry. B 114(11): 4010-4019, 2010

Network formation studied by temperature scanning Brillouin scattering and differential scanning calorimetry techniques. II. Journal of Polymer Science. Part B. Polymer Physics 39(12): 1326-1336, 2001

Differential scanning calorimetry study of the thermodynamic stability of some mutants of Sso7d from Sulfolobus solfataricus. Biochemistry 37(29): 10493-10498, 1998

Does thermodynamic stability of peritoneal collagen change during laparoscopic cholecystectomies? a differential scanning calorimetry (DSC) study. Surgical Endoscopy 28(9): 2623-2626, 2014

Thermodynamic Characterization of Polymorphs in Bulk-Crystallized Syndiotactic Polystyrene via Small/Wide-Angle X-ray Scattering and Differential Scanning Calorimetry. Macromolecules 42(12): 4200-4207, 2009

New class of aggregates in aqueous solution: an NMR, thermodynamic, and dynamic light scattering study. Langmuir: the Acs Journal of Surfaces and Colloids 22(14): 6031-6041, 2006

Thermal hysteresis in ganglioside micelles investigated by differential scanning calorimetry and light-scattering. Langmuir 15(15): 4975-4980, 1999