+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Thermotolerance and heat-shock protein expression in Northeastern Pacific Nucella species with different biogeographical ranges



Thermotolerance and heat-shock protein expression in Northeastern Pacific Nucella species with different biogeographical ranges



ine Biology Berlin il; 146(5): 985-993



We investigated physiological traits responsible for determining the tide-height and latitudinal distributions of Northeastern Pacific Nucella congeners. First, we determined the thermotolerances of two species of intertidal dogwhelks, N. ostrina and N. canaliculata, which co-occur on the Oregon coast. We found that N. ostrina, which are distributed higher on the shore, and thus experience higher habitat temperatures, than N. canaliculata, had correspondingly higher heat-coma temperatures. Second, we acclimated individuals of all five Northeastern Pacific Nucella congeners to a common temperature and determined their thermotolerances, measured as recovery from thermal exposure, after a 5-day, 3-week, and 7-week acclimation period. The south-latitude (N. emarginata) and mid-latitude (N. ostrina) high-intertidal species were more thermotolerant than the mid-latitude low-intertidal (N. canaliculata and N. lamellosa) and north-latitude high-intertidal (N. lima) species. The results of these two experiments suggest that temperature plays a role in determining the tide-height and latitudinal distributions of these Nucella species. Finally, we measured total and inducible levels of an evolutionarily conserved and ecologically relevant protein, the 70-kDa heat-shock protein (Hsp70), which has been found to confer thermotolerance in model laboratory organisms. The results showed that the level of total, not stress inducible, Hsp70 was a better predictor of thermotolerance and that there were species-specific differences in the relationship between Hsp70 expression and thermotolerance. We suggest that Hsp70 expression may be important in conferring thermotolerance in Nucella species in nature and that higher levels of molecular chaperones may underlie increased thermotolerance between conspecifics.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012690818

Download citation: RISBibTeXText

DOI: 10.1007/s00227-004-1508-2


Related references

Heat-shock protein expression in Nucella species of the Northeastern Pacific. American Zoologist 41(6): 1592, 2001

Complex patterns of expression of heat-shock protein 70 across the southern biogeographical ranges of the intertidal mussel Mytilus californianus and snail Nucella ostrina. Journal of Biogeography il; 33(4): 622-630, 2006

Effects of cycloheximide on thermotolerance expression heat shock protein synthesis and heat shock protein messenger rna accumulation in rat fibroblasts. Molecular and Cellular Biology 6(4): 1088-1094, 1986

Expression of heat shock protein 70 and heat shock protein 27 in lens epithelial cells induced by contusion and thermotolerance in rat model. Chinese Journal of Ophthalmology 42(3): 241-245, 2006

Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation in rat fibroblasts. Molecular and Cellular Biology 6(4): 1088-1094, 1986

Effects of cycloheximide on heat shock protein synthesis heat shock rna accumulation and thermotolerance expression in mammalian cells. Journal of Cellular Biochemistry Suppl. (9 Part B): 248, 1985

Induced thermotolerance and the heat shock protein-70 family in the Pacific oyster Crassostrea gigas. Molecular Marine Biology and Biotechnology 7(1): 21-30, 1998

Variation in the heat shock response and its implication for predicting the effect of global climate change on species' biogeographical distribution ranges and metabolic costs. Journal of Experimental Biology 213(6): 971-979, 2010

Heat-shock protein 104 expression is sufficient for thermotolerance in yeast. Proceedings of the National Academy of Sciences of the United States of America 93(11): 5301-5306, 1996

Dissociation of 68,000 Mr heat shock protein synthesis from thermotolerance expression in rat fibroblasts. Radiation Research 99(2): 433-437, 1984

Molecular identification and expression of heat shock cognate 70 and heat shock protein 70 genes in the Pacific oyster Crassostrea gigas. Cell Stress and Chaperones 8(1): 76-85, 2003

Species-specific collaboration of heat shock proteins Hsp 7 and 1 in thermotolerance and protein disaggregation. Proceedings of the National Academy of Sciences 108(17): 6915-6920, 2011

Species-specific collaboration of heat shock proteins (Hsp) 70 and 100 in thermotolerance and protein disaggregation. Proceedings of the National Academy of Sciences of the United States of America 108(17): 6915-6920, 2011

Molecular identification and expression of heat shock cognate 70 (hsc70) and heat shock protein 70 (hsp70) genes in the Pacific oyster Crassostrea gigas. Cell Stress and Chaperones 8(1): 76-85, 2003

Induction of thermotolerance in chickens by temperature conditioning: heat shock protein expression. Annals of the new York Academy of Sciences 813: 628-636, 1997