+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Thrombin, kallikrein and complement C5b-9 adsorption on hydrophilic and hydrophobic titanium and glass after short time exposure to whole blood

Thrombin, kallikrein and complement C5b-9 adsorption on hydrophilic and hydrophobic titanium and glass after short time exposure to whole blood

Biomaterials 25(16): 3171-3176

Hydrophilic and hydrophobic titanium and glass were exposed to capillary whole blood between 5s and 24h. The time-sequence for adsorption of thrombin, kallikrein and complement C5b-9, and their relationship with adherent platelets and polymorphonuclear granulocyte (PMN) activation were investigated. Adsorbed thrombin and kallikrein were measured by cleavage of specific chromogenic substances, S-2238 and S-2303, respectively. Complement C5b-9 and expression of CD11b, CD66b, CD62P and Pan-platelets were measured by immunofluorescence. Thrombin and kallikrein were present on the surfaces during the whole investigated periods. Platelet adhesion and PMN cell adhesion and activation on all surfaces and activation of platelets on hydrophobic surfaces showed a similar pattern to thrombin adsorption. Kallikrein adsorption had a different pattern on each surface. C5b-9 was detected between 32min and 24h of blood exposure and a varying pattern of C5b-9 coverage was observed on each surface. In conclusion, our results indicate that the interaction between material and blood coagulation and kinin-activating proteins regulate the adhesion and activation of blood cells, whereas after longer time the coagulation and kallikrein-kinin system play minor roles and the complement system is decisive for mediating and elongating the inflammatory process.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 012693175

Download citation: RISBibTeXText

PMID: 14980412

DOI: 10.1016/j.biomaterials.2003.10.011

Related references

Platelet binding and protein adsorption to titanium and gold after short time exposure to heparinized plasma and whole blood. Biomaterials 17(23): 2225-2232, 1996

Direct real-time imaging of protein adsorption onto hydrophilic and hydrophobic surfaces. Biopolymers 93(1): 74-84, 2010

Polymorphonuclear leukocytes in coagulating whole blood recognize hydrophilic and hydrophobic titanium surfaces by different adhesion receptors and show different patterns of receptor expression. Journal of Laboratory and Clinical Medicine 137(4): 296-302, 2001

Adsorption of thrombin to glass surfaces and changes of thrombin activity. Yakugaku Zasshi 103(4): 411-413, 1983

Adsorption of trypsin on hydrophilic and hydrophobic surfaces. Langmuir 23(4): 2000-2006, 2007

Adsorption of iga from saliva to hydrophobic and hydrophilic substrata. Journal of Dental Research 68(SPEC ISSUE JUNE): 984, 1989

Recyclable hydrophilic-hydrophobic micropatterns on glass for microarray applications. Langmuir 23(9): 4728-4731, 2007

Adsorption of hydrophobin proteins at hydrophobic and hydrophilic interfaces. Colloids and Surfaces. B Biointerfaces 44(4): 172-178, 2005

Biomechanic and histomorphometric studies of HIP titanium glass ceramic, a new implant material, compared with glass ceramics, titanium and titanium alloy. Deutsche Zeitschrift für Mund- Kiefer- und Gesichts-Chirurgie 14(1): 53-60, 1990

New synthetic inhibitors of activated complement c 1r esterase complement c 1 esterase thrombin plasmin kallikrein and trypsin. Biochimica et Biophysica Acta 661(2): 342-345, 1981

Effect of water on the adsorption of hydrocarbons on a hydrophilic or hydrophobic surface. Abstracts of Reports - International Congress on Organic Geochemistry 6, Advances in organic geochemistry 1973): 925-938, 1974

Adsorption of non-ionic surfactants on hydrophobic and hydrophilic carbon surfaces. Journal of Colloid and Interface Science 343(1): 194-199, 2010

Osteogenic response of human MSCs and osteoblasts to hydrophilic and hydrophobic nanostructured titanium implant surfaces. Journal of Biomedical Materials Research. Part a 104(12): 3137-3148, 2016

Implantation of hydrophilic and hydrophobic titanium discs in rat tibia: cellular reactions on the surfaces during the first 3 weeks in bone. Biomaterials 25(19): 4759-4766, 2004

Adsorption of fibrinogen on silicon oxide with controlled hydrophobic/hydrophilic properties. Biofizika 34(4): 557-560, 1989