+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Thymidine analogs are transferred from prelabeled donor to host cells in the central nervous system after transplantation: a word of caution



Thymidine analogs are transferred from prelabeled donor to host cells in the central nervous system after transplantation: a word of caution



Stem Cells 24(4): 1121-1127



Thymidine analogs, including bromodeoxyuridine, chlorodeoxyuridine, iododeoxyuridine, and tritiated thymidine, label dividing cells by incorporating into DNA during S phase of cell division and are widely employed to identify cells transplanted into the central nervous system. However, the potential for transfer of thymidine analogs from grafted cells to dividing host cells has not been thoroughly tested. We here demonstrate that graft-derived thymidine analogs can become incorporated into host neural precursors and glia. Large numbers of labeled neurons and glia were found 3-12 weeks after transplantation of thymidine analog-labeled live stem cells, suggesting differentiation of grafted cells. Remarkably, however, similar results were obtained after transplantation of dead cells or labeled fibroblasts. Our findings reveal for the first time that thymidine analog labeling may not be a reliable means of identifying transplanted cells, particularly in highly proliferative environments such as the developing, neurogenic, or injured brain.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012693666

Download citation: RISBibTeXText

PMID: 16373692

DOI: 10.1634/stemcells.2005-0463


Related references

Adoptively transferred experimental allergic encephalomyelitis in chimeric rats: identification of transferred cells in the lesions of the central nervous system. Immunology 65(1): 23-29, 1988

Mast cells of the mammalian central nervous system 6. uptake of tritium labeled thymidine by mast cells neuro lipo mastocytoid cells and other elements of the central nervous system. Acta Anatomica 108(1): 102-114, 1980

Donor-derived cells in the central nervous system of twitcher mice after bone marrow transplantation. Science 239(4843): 1035-1038, 1988

Donor derived cells are present in the central nervous system cns of twitcher mice after bone marrow transplantation bmt. Experimental Hematology 16(6): 533, 1988

The mast cells of the mammalian central nervous system. VI. Uptake of tritiated thymidine by mast cells, neurolipomastocytoid cells and other elements of the central nervous system. Acta Anatomica 108(1): 102-114, 1980

Donor-recipient size matching in pediatric heart transplantation A word of caution about small grafts. Journal of Heart & Lung Transplantation 14(1 Part 2): S48, 1995

Infections of the Central Nervous System after Unrelated Donor Umbilical Cord Blood Transplantation or Human Leukocyte Antigen-Matched Sibling Transplantation. Biology of Blood and Marrow Transplantation 23(1): 134-139, 2017

Donor-derived infections with central nervous system pathogens after solid organ transplantation. JAMA 310(4): 378-379, 2013

Accumulation of passively transferred primed T cells independently of their antigen specificity following central nervous system trauma. Journal Of Neuroimmunology. 89(1-2): 88-96,. 14, 1998

Reduction of fatal graft vs host disease by tritiated thymidine suicide of donor cells cultured with host cells. Transplantation 23(4): 299-302, 1977

Reduction of fatal graft-versus-host disease by 3H-thymidine suicide of donor cells cultured with host cells. Transplantation 23(4): 299-302, 1977

Malignancy transplantation with graft: do patients with primary central nervous system tumors have to be excluded from the donor pool?. Transplant International 10(1): 83-84, 1997

The Rate of Tritiated Thymidine Incorporation by Lymphoid Cells Transferred to a Heterologous Host. Israel Medical Journal 23: 153-161, 1964

Ganciclovir-sensitive acute graft-versus-host disease in mice receiving herpes simplex virus-thymidine kinase-expressing donor T cells in a bone marrow transplantation setting. Transplantation 69(4): 503-508, 2000