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Toxicity of Bt toxic protein expressed by cry1Aa cloned in Escherichia coli



Toxicity of Bt toxic protein expressed by cry1Aa cloned in Escherichia coli



Sichuan Daxue Xuebao (Ziran Kexueban) 41(1): 193-197



The cultural characteristics and toxicity of Bt cry1Aa gene cloned in Escherichia coli (ECE52) were studied. The results indicated that the optimal cultural condition for this engineered bacterium was 8apprx12h at 37C. Toxicity tests showed that the crystal protein expressed by ECE52 possessed high toxicity to the fourth instars larvae of silkworm. The mortality of larvae was 100% in 96h when fresh leaves treated by 62.5 X 10-6g/mL ECE52 were provided as food for 12h.

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