+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection

, : Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection. Nature Structural & Molecular Biology 12(1): 67-74

The conjugation of small ubiquitin-like modifiers SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase. NMR chemical shift perturbation was used to identify the surface of Ubc9 that interacts with the SUMO ligase RanBP2. Unlike known ubiquitin E2-E3 interactions, RanBP2 binds to the beta-sheet of Ubc9. Mutational disruption of Ubc9-RanBP2 binding affected SUMO-2 but not SUMO-1 conjugation to Sp100 and to a newly identified RanBP2 substrate, PML. RanBP2 contains a binding site specific for SUMO-1 but not SUMO-2, indicating that a Ubc9-SUMO-1 thioester could be recruited to RanBP2 via SUMO-1 in the absence of strong binding between Ubc9 and RanBP2. Thus we show that E2-E3 interactions are not conserved across the ubiquitin-like protein superfamily and identify a RanBP2-dependent mechanism for SUMO paralog-specific conjugation.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 012722700

PMID: 15608651

DOI: 10.1038/nsmb878

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Higgins, D. A.; Shortridge, K. F.; Ng, P. L. K., 1987: Bile immunoglobulin of the duck anas platyrhynchos ii. antibody response in influenza a virus infections. The capacity of the IgM-like bile immunoglobulin (IgX) of the duck (Anas platyrhynchos) to express antibody activity to H3N2 influenza A viruses, and the dependence of this activity on the co-existence of serum IgM antibodies were investigated. Du...

Theisen, Michael, 1996: Molecular cloning and characterization of nlpH, encoding a novel, surface-exposed, polymorphic, plasmid-encoded 33-kilodalton lipoprotein of Borrelia afzelii. Borrelia burgdorferi sensu lato organisms, comprising B. burgdorferi sensu stricto, Borrelia afzelii, and Borrelia garinii, are tick-borne pathogens causing Lyme borreliosis in humans. To identify putative virulence determinants, a B. afzelii DNA...

Omori, Yasufumi; Dagli, Maria Lucia Zaidan; Yamakage, Kohji; Yamasaki, Hiroshi, 2001: Involvement of gap junctions in tumor suppression: Analysis of genetically-manipulated mice. Accumulating evidence indicates that gap junctions play an important role in the maintenance of normal cell growth, so that genes for the connexin gap junction proteins form a family of tumor-suppressor genes. Although mice from which nine types o...

Rind, D., 1987: Predicting regional climate change: improving the models. Greenhouse effect climate change and US forests edited by William E Shands and John S Hoffman: 0

Nechitailo, V. A., 1959: Hyperplastic processes in the bone marrow in acute leukemias. Arkhiv Patol 21(11): 73-78

Danielewicz, B.; Finley, K.; Kingsley, M.; Marko, J.; Richwa, P.; Schweinsburg, R.; Smith, T.; Stirling, I.; Webb, T., 1986: Beaufort Environmental Monitoring Project 1984-1985 Hypothesis No 5 Icebreaker traffic in the transition shear zone will reduce bearded seal pup production. Environmental Studies (Ottawa): 39-41 No. 39

Thertulien, Raymond; Ojha, Rohit; Zangari, Maurizio; Fassas, Athanasios; Anaissie, Elias J.; Lee, Choon Kee; Vanrhee, Frits; Barlogie, Bart; Tricot, Guido J., 2003: Myelodysplastic CD34+ clones can be detected by fluorescent in situ hybridization in the stem cell harvest of myeloma patients who develop treatment-related myelodysplastic syndrome following autologous transplantation. Treatment-related myelodysplasia (t-MDS) is a well-recognized late complication of autologous transplant (AT). However, controversy remains regarding the contribution of prior chemotherapy and high dose therapy to the development of post-transplan...

Biermann, Heike; Pietz, Barbara; Dreier, Rita; Schmid, Kurt W.; Sorg, Clemens; Sunderkoetter, Cord, 1999: Murine leukocytes with ring-shaped nuclei include granulocytes, monocytes, and their precursors. Leukocytes with ring-shaped nuclei (ring cells) are present in bone marrow (BM; apprx50% of BM cells), in peripheral blood (PB), and in inflammatory infiltrates of mice, but also in humans during myeloproliferative disorders. They are usually refe...

Luck, H., 1975: Determination of the fat protein and lactose content of 10 day preserved milk samples by means of the ir milk analyzer. The IRMA unit can be used to determine the fat, protein and lactose content of 10-day preserved milk samples (preserved with 0.3% potassium dichromate or 0.3% mercuric chloride). Virtually the same mean constituent concentrations were measured on...

Wang C.F.; Gemzell C., 1982: Neocept a simple sensitive urine test of early pregnancy in women undergoing ovulation induction. Fifty-five anovulatory women undergoing hMG-[human menopausal gonadotropin] hCG [human chorionic gonadotropin] treatment in order to become pregnant had daily first morning urine specimens and blood samples drawn, starting from the 11th postovulat...