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Enzymatic acidolysis of evening primrose oil with docosahexaenoic acid using response surface methodology



Enzymatic acidolysis of evening primrose oil with docosahexaenoic acid using response surface methodology



Journal of food lipids 13(3): 235-250



Lipase-catalyzed acidolysis of evening primrose oil with docosahexaenoic acid (DHA) was carried out using Novozym-435 from Candida antarctica as the biocatalyst. Several parameters, namely the amount of enzyme (X subscript 1(B; 100-200 enzyme activity units), incubation temperature (X subscript 2(B; 30-60C) and reaction time (X subscript 3(B; 18-30 h) were examined as independent variables of face-centered cube design. Using response surface methodology (RSM), the optimum reaction conditions for DHA incorporation into evening primrose oil were determined and quadratic response surface was drawn from the mathematical model. The results demonstrated that the amount of enzyme, reaction temperature and reaction time significantly (P <= 0.05) affected the incorporation of DHA into evening primrose oil. Optimum reaction conditions for DHA incorporation were 162 units of enzyme at 43C in 27 h. The optimum value predicted by RSM for DHA incorporation was 31.9%. Analysis of variance showed that 95% (R superscript 2(B = 0.95) of the observed variation was explained by the polynomial model. Close agreement existed between experimental and predicted values. The positional distribution of fatty acids in DHA-enriched evening primrose indicated that DHA was randomly distributed among the sn-2 and sn-1 + sn-3 positions of the DHA-enriched evening primrose oil.

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Accession: 012786005

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DOI: 10.1111/j.1745-4522.2006.00048.x


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