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Establishment and application of a polymerase chain reaction-based method to detect duck plague virus (DPV) attenuated vaccine strain and virulent strain



Establishment and application of a polymerase chain reaction-based method to detect duck plague virus (DPV) attenuated vaccine strain and virulent strain



Chinese Journal of Veterinary Medicine 41(2): 17-20



In an experiment, optimized conditions for polymerase chain reaction-based method to detect DPV (duck plague virus) were defined as follows: re-warming temperature of 51.8 degrees C, working concentration of 2.5 micro mol for magnesium ion, working concentration of 200 micro mol for dNTP, and 35 cycles in all.

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