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Establishment of ISSR reaction system of Sargentodoxa cuneata



Establishment of ISSR reaction system of Sargentodoxa cuneata



Acta Agriculturae Universitatis Jiangxiensis 28(4): 583-586



The genomic DNA of S. cuneata was extracted using an improved SDS method and a suitable reaction system for the intersimple sequence repeat (ISSR) amplification in the crop was optimized. A optimum PCR reaction was composed of 4X Taq polymerase, 0.5U Taq DNA polymerase, 2 mmol MgCl2/litre, 0.1 mol 4X dNTP/litre, 12 pmol primer, 10 ng template DNA and 2 mg BSA/litre in a total reaction volume of 10 micro l.

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