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Establishment of bovine mammary epithelial cell culture system



Establishment of bovine mammary epithelial cell culture system



Xinjiang Agricultural Sciences 43(5): 394-398



A cell culture system for bovine mammary epithelial cells (BMEC) from milk was established to study the proliferation, differentiation and gene expression in mammary epithelial cells. The basic culture medium is DMEM/f12 (v/v=1:1), 20% fetal bovine serum and insulin (5 micro g/ml), with addition of transferrin (5 micro g/ml), progesterone (1 micro g/ml), penicillin (100 IU/ml) and streptomycin (100 IU/ml). The cells were cultured on plastic Petri dishes and were covered with collagen.

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