+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Establishment of in vitro cultivation of Giardia canis trophozoites infected with Giardia canis virus

Establishment of in vitro cultivation of Giardia canis trophozoites infected with Giardia canis virus

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 24(4): 261-265

Objective To cultivate a Giardia canis isolate with G. canis virus (GCV). Methods Five-day-old Meriones unguiculatus was infected with the cysts of G. canis isolated from dogs in Changchun and purified by sucrose density gradient centrifugation-G1 acid funnel filtration method. Trophozoites were isolated aseptically from the duodenum of the infected rodent after 8 days, then transferred to modified TYI-S-33 medium and cultivated at 37[degree]C. The trophozoites were centrifuged with 3 000 x g. 15 min after liquid nitrogen freeze-thawing three times and the supernatant stained negatively by phosphotungstic acid was observed with transmission electron microscope. Results G. canis trophozoites which adapted gradually to the environment and grew a cellular monolayer after 14 (lays were examined by freezing and thawing experiment, purity quotient, stability, biology characteristics and microbial contamination detection. The results demonstrated that a stable G.canis trophozoite cell isolate was established. G. canis virus with icosahedron spherical shape and 36 nm in diameter was observed by electron microscope. Conclusion In vitro cultivation of G. canis trophozoites with GCV is established.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 012786392

Download citation: RISBibTeXText

PMID: 17094593

Related references

Giardia canis: ultrastructural analysis of G. canis trophozoites transfected with full length G. canis virus cDNA transcripts. Experimental Parasitology 123(3): 212-217, 2009

Ultrastructure of Giardia canis trophozoites. Zentralblatt Fuer Veterinaermedizin Reihe B: 312: 107-114, 1984

Inactivation of Giardia lamblia and Giardia canis cysts by combined and free chlorine. Applied and Environmental Microbiology 54(10): 2580-2582, 1988

Axenic cultivation of Giardia canis. Chinese Journal of Zoonoses ; 21(8): 706-709, 2005

Recognition of a 30,000 MW antigen of Giardia muris trophozoites by intestinal IgA from Giardia-infected mice. Immunology 70(4): 535-539, 1990

Giardia infection in pigs: detection and in vitro isolation of trophozoites of the Giardia intestinalis group. Parasitology 102 Pt 2: 163-166, 1991

In vitro cultivation of Giardia trophozoites. Giardia and giardiasis biology, pathogenesis, and epidemiology: 99-109, 1984

In vitro cultivation of Giardia trophozoites. Giardiasis : 99-110, 1990

Dietary lipids containing gangliosides reduce Giardia muris infection in vivo and survival of Giardia lamblia trophozoites in vitro. Parasitology 128(Pt 6): 595-602, 2004

Identification and characterization of Giardia canis dsRNA virus. Chinese Journal of Veterinary Science 21(5): 475-478, 2001

Isolation of Giardia from Swiss cattle and cultivation of trophozoites in vitro. Parasitology Research 73(2): 182-183, 1987

Giardia canis hegner 1922 i. distribution in the digestive tract of naturally infected dogs. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 39(2): 265-272, 1987

The influence of anti giardia antibody heat labile serum components and sensitized host cells on short term in vitro interactions between giardia lamblia trophozoites and rat peritoneal leukocytes. Abstracts of the Annual Meeting of the American Society for Microbiology 84: ABSTRACT E53, 1984

Effect of cytochalasin-B, low Ca++ calcium ion concentration, iodoacetic acid, and quinacrine-HCl hydrogen chloride on the attachment of Giardia trophozoites in vitro Rats, protozoal infections, parasites, antiprotozoal agents, Giardia spp. p. 1982

Evaluation of a new method for in vitro cultivation of giardia lamblia trophozoites in the diagnosis of giardiasis. Abstracts of the Annual Meeting of the American Society for Microbiology 85: 302, 1985