+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Estimation of ochratoxin A in portuguese population: new data on the occurrence in human urine by high performance liquid chromatography with fluorescence detection



Estimation of ochratoxin A in portuguese population: new data on the occurrence in human urine by high performance liquid chromatography with fluorescence detection



Food and Chemical Toxicology 44(9): 1449-1454



With increasing knowledge of the persistence of OTA in the food chain, exposure to this mycotoxin is a potential human health hazard to humans, and evaluating its presence in populations has become highly important. A sensitive and accurate analytical method for the determination of ochratoxin A in urine was validated, since is less invasive than blood monitoring. It involves extraction with 5% NaHCO3, immunoaffinity column (IAC) for clean-up and high performance liquid chromatography with fluorescence detection (HPLC-FD). The limit of quantification was 0.02 ng/mL of urine (1.3 ng/mL of the extract injected) and recovery of ochratoxin A from urine samples spiked at the three fortification levels, were higher than 90% with RSD lower than 9%. The identification of OTA was confirmed by methyl ester derivatization and then HPLC analysis. Based in ours first results we can assume that OTA conjugation with glucuronic acid in human urine occurs. In the present study, we follow up OTA levels in 60 urine samples of inhabitants from Coimbra city, Portugal, in order to evaluate population contamination, and the presence of OTA was found in 42 samples, at concentrations above the LOQ, ranged between 0.021 and 0.105 ng/mL.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012786649

Download citation: RISBibTeXText

PMID: 16806627

DOI: 10.1016/j.fct.2006.04.017


Related references

Determination of ochratoxin A in Portuguese rice samples by high performance liquid chromatography with fluorescence detection. Analytical and Bioanalytical Chemistry 382(5): 1288-1293, 2005

Determination of ochratoxin A at the ppt level in human blood, serum, milk and some foodstuffs by high-performance liquid chromatography with enhanced fluorescence detection and immunoaffinity column cleanup: Methodology and Swiss data. Journal of Chromatography B Biomedical Applications 666(1): 85-99, 1995

Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry. Journal of Analytical Toxicology 14(2): 123-126, 1990

Melatonin determination in human urine by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography 377: 350-355, 1986

Determination of 21-hydroxycorticosteroids in human urine by high-performance liquid chromatography with fluorescence detection. Chemical and Pharmaceutical Bulletin 37(11): 3022-3025, 1989

Enantioselective high-performance liquid chromatography with fluorescence detection of methamphetamine and its metabolites in human urine. Analyst 123(11): 2333-2337, 1999

Determination of bumetanide in human plasma and urine by high performance liquid chromatography with fluorescence detection. Analytical Letters 21(9): 1589-1602, 1988

Determination of Bumetanide in Human Plasma and Urine by High-Performance Liquid Chromatography with Fluorescence Detection. Analytical Letters 21(9): 1589-1601, 1988

Determination of norfloxacin in human plasma and urine by high-performance liquid chromatography and fluorescence detection. Journal Of Chromatography A. 812(1-2): 381-385, Y 3, 1998

Determination of ethambutol in human plasma and urine by high-performance liquid chromatography with fluorescence detection. Journal Of Chromatography A. 729(1-2): 301-307, 1996

Determination of pivaloylcarnitine in human plasma and urine by high-performance liquid chromatography with fluorescence detection. Journal Of Pharmaceutical Sciences. 81(10): 1038-1041, 1992

Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography B 760(2): 207-212, 5 September, 2001

Determination of metoprolol in human plasma and urine by high-performance liquid chromatography with fluorescence detection. Journal of Separation Science 33(13): 1904-1908, 2010

Determination of debrisoquine and 4 hydroxydebrisoquine in human urine by high performance liquid chromatography and fluorescence detection. Journal of Liquid Chromatography 13(8): 1627-1636, 1990

Determination of baclofen in human plasma and urine by high-performance liquid chromatography with fluorescence detection. Analyst 120(2): 373-375, 1995