+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Eukaryotic expression of HA1 gene of H5 avian influenzavirus in insect cells and the antigenicity of the expressed protein



Eukaryotic expression of HA1 gene of H5 avian influenzavirus in insect cells and the antigenicity of the expressed protein



Veterinary Science in China 36(5): 352-356



HA1 gene of the H5 avian influenzavirus (AIV) was expressed in insect cells by pBlueBacHis2 baculovirus vector. Western-blotting analysis revealed that the expressed HA1 protein had antigens, but did not react with H7 and H9 AIV anti-serum. Dot-ELISA analysis showed that the expressed recombinant protein had special characteristics under non-denaturing conditions.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 012786813

Download citation: RISBibTeXText


Related references

Expression of HA1 gene of avian influenza virus H7 subtype in recombinant baculovirus and analysis of antigenicity of the expressed HA1 protein. Veterinary Science in China 37(3): 232-235, 2007

Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system. Biotechnology Letters 34(9): 1623-1627, 2013

Expression of NP gene of highly pathogenic avian influenza virus subtype H5N1 in insect cells and identification of biological activities of the expressed product. 2008

Construction of eukaryotic expression vector and expression of outer membrane protein H gene of avian Pasteurella multocida C48-1 and chicken interkine-18 gene. Chinese Journal of Veterinary Science 26(4): 382-384, 2006

Prokaryotic expression of bovine herpesvirus-1 gB gene and the antigenicity of the expressed protein. Veterinary Science in China 36(7): 523-528, 2006

Prokaryotic expression of vp1 gene of goose parvovirus and antigenicity analysis of the expressed protein. Chinese Veterinary Science: 2, 135-138, 2010

Cloning and expression of pEtK2 gene of Eimeria tenella and antigenicity of the expressed protein. Zhongguo Shouyi Kexue: 200-204 Sum No 355, 2007

Expression of overlapping fragments of BVDV p80 gene and analysis of antigenicity of expressed protein. Veterinary Science in China 36(4): 257-261, 2006

Cloning and expression of protein fiber1 gene of chicken embryo lethal orphan virus and identification of expressed protein antigenicity. Chinese Journal of Veterinary Science and Technology 35(6): 468-471, 2005

Construction and transient expression of recombinant avian influenzavirus with nucleoprotein gene. Veterinary Science in China 36(3): 189-192, 2006

Expression of avian infectious bronchitis virus nucleocapsid protein gene in E. coli and the study of its antigenicity. Chinese Journal of Virology 19(4): 342-347, 2003

Prokaryotic expression of NS1 gene of H5N1 avian influenzavirus and development of direct ELISA. Chinese Journal of Veterinary Science and Technology 35(8): 585-589, 2005

Expression of haemagglutinin gene of avian influenzavirus subtype H5 in Escherichia coli and the preliminary analysis of bioactivity. Journal of Agricultural Biotechnology 13(6): 739-742, 2005

Detection of antibodies against avian influenza virus by protein microarray using nucleoprotein expressed in insect cells. Journal of Veterinary Medical Science 77(4): 413-419, 2016

Expression of tick-borne encephalitis virus prM-E protein in insect cells and studies on its antigenicity. Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 19(4): 335-339, 2006