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Eukaryotic expression of the VP1 gene of foot-and-mouth disease virus type O and characterization of expressed fusion protein



Eukaryotic expression of the VP1 gene of foot-and-mouth disease virus type O and characterization of expressed fusion protein



Veterinary Science in China 36(3): 183-188



A VP1 gene in the plasmid pMD18-T-VP1 was subcloned into an eukaryotic vector pBlueBacHis2A to construct a recombinant plasmid pBlueBacHis2A-VP1 (633 bp). The constructed recombinant plasmid and Bac-N-BlueTM DNA were then co-transfected into sf9 cells. After screening the plaques, sf9 cells were infected with the recombinant virus which produced the 32.6 ku target protein.

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