+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of 10 commercial diagnostic kits for in vitro expressed hepatitis B virus (HBV) surface antigens encoded by HBV of genotypes A to H



Evaluation of 10 commercial diagnostic kits for in vitro expressed hepatitis B virus (HBV) surface antigens encoded by HBV of genotypes A to H



Journal of Virological Methods 136(1-2): 254-256



Genetic variability of the hepatitis B virus (HBV) constitutes one of the major challenges for diagnosis of HBV infection. It is plausible that amino acid substitutions in the "a" determinant of the HBV surface antigen (HBsAg) that affect antigenic sites, whether originating from genetic diversity or from mutations in the HBV strain itself, will affect the sensitivity of some diagnostic kits. In fact, recent studies have indicated that some diagnostic kits had false negative results with particular HBsAg mutants. There have been, however, few substantial studies evaluating sensitivities of diagnostic kits to the HBsAg encoded by different HBV genotypes. Our recent study found that 10 diagnostic kits available in Japan were able to detect HBsAg irrespective of whether it originated from HBV genotypes A, B or C, with the latter two genotypes being the dominant species in East Asia. In this study, we extended our previous efforts by assessing the ability of diagnostic kits to detect recombinant HBsAg derived from HBV genotypes A to H. Our results demonstrated that 9 out of 10 diagnostic kits evaluated were able to detect as low as 0.2 International Units (IU)/ml HBsAg, irrespective of HBV genotype. The genotypic differences in the HBV family thus appear to have little impact on the sensitivity of currently available HBsAg diagnostic kits.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012786959

Download citation: RISBibTeXText

PMID: 16707169

DOI: 10.1016/j.jviromet.2006.03.022


Related references

Reactivity of genotypically distinct hepatitis B virus surface antigens in 10 commercial diagnostic kits available in Japan. Japanese Journal of Infectious Diseases 58(2): 83-87, 2005

Evaluation of in vitro screening and diagnostic kits for hepatitis C virus infection. Journal of Clinical Virology 105: 97-102, 2018

Reactivity of 13 in vitro expressed hepatitis B surface antigen variants in 7 commercial diagnostic assays. Hepatology 31(5): 1176-1182, 2000

Assay of hepatitis b virus dna by polymerase chain reaction and its relationship to pre s encoded and s encoded viral surface antigens. Hepatology 13(1): 158-166, 1991

Enzyme-linked immunoassay for hepatitis B surface antigen. An evaluation of commercial test kits. Transfusion 23(1): 45-48, 1983

Evaluation of the ELISA diagnostic kits for hepatitis E virus antibody in the reference serum, the suspect patients of hepatitis E and normal persons' sera. Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 21(1): 59-61, 2007

Comparative evaluation of diagnostic kits for detection of antibodies to hepatitis C virus. Voprosy Virusologii 41(2): 61-63, 1996

Evaluation on the anti-hepatitis C virus enzyme-linked immunosorbent diagnostic kits. Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 12(2): 115-117, 2003

Hepatitis B virus surface antigen mutant associated to lamivudine therapy shows decreased reactivity to diagnostic kits. Journal of Gastroenterology & Hepatology 17(Supplement): A1080, December, 2002

Diagnostic usefulness of antineutrophil cytoplasmic autoantibody serology. Comparative evaluation of commercial indirect fluorescent antibody kits and enzyme immunoassay kits. American Journal of Clinical Pathology 111(3): 363-369, 1999

Assay of hepatitis B virus DNA by polymerase chain reaction and its relationship to pre-S- and S-encoded viral surface antigens. Hepatology 13(1): 158-166, 1991

Assays for detecting hepatitis B virus envelope antigens or antibodies thereto and diagnostic test kits for use in performing the assays. Official Gazette of the United States Patent & Trademark Office Patents 1197(3): 1861, April 15, 1997

Nonoverlapping antigenic sites of woodchuck hepatitis virus surface antigen and their cross-reactivity with ground squirrel hepatitis virus and hepatitis B virus surface antigens. Journal of Virology 47(1): 15-23, 1983

Establishment of the evaluation reference system for domestic anti-hepatitis C virus diagnostic enzyme immunoassay kits. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 29(7): 761-764, 2013

Hepatitis B virus genotypes and hepatitis B surface antigen mutations in family contacts of hepatitis B virus infected patients with occult hepatitis B virus infection. Journal of Gastroenterology and Hepatology 24(4): 588-598, 2009