Transient gus expression studies in onion (Allium cepa L.) and garlic (Allium sativum L.)

Anil Khar; Yadav, R.C.; Neelam Yadav; Bhutani, R.D.

Ziraat Fakultesi Dergisi, Akdeniz Universitesi 18(3): 301-304

2005


Accession: 012829838

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Abstract
Transient gus expression was evaluated using onion cultivars B-780, N-2-4-1 and Hisar 2, and garlic cultivars HG-1, G-41 and G-1. The effects of 2 plasmids (pCAMBIA 1301 and pTOK 233) on the genetic transformation was determined on callus, shoot tip and root explants of onion, and on root, basal portion and young etiolated leaves of garlic. The suitability of the explants for transformation was also evaluated. Onion callus from different sources was superior to shoot tips. No gus expression was obtained in onion root tips. In garlic, only the basal portions showed gus expression, and leaves and roots did not show any response. Plasmid pCAMBIA 1301 was superior to pTOK 233 in transforming the plants. No significant variation was observed among genotypes.