+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Characterization of wax esters, free fatty alcohols and free fatty acids of crude wax from sunflower seed oil refineries

Characterization of wax esters, free fatty alcohols and free fatty acids of crude wax from sunflower seed oil refineries

Food chemistry01(4): 1552-1557

The sunflower seed wax (10-12%) from oil refineries was purified through two steps, namely, extraction using solvents and precipitation with chilled acetone. Fatty esters, free fatty alcohols and free fatty acids were separated by thin-layer chromatography (TLC). The fractions of fatty esters and free fatty alcohols were separated and identified by gas chromatography (GC) using a Dexil-300 column and OV-101 column, respectively, at temperatures from 150-290 pC and were further confirmed by Gas chromatography-mass spectrometry (GC-MS), using a capillary silica column SPB-1 coated with polydimethyl siloxane. Among the long chain fatty esters (C38-C54), comprised of one unit of fatty alcohol and one unit of fatty acid through an ester linkage, the major esters were C40-C44, of which C42 was predominant. These fatty esters, upon hydrolysis, followed by methylation and subsequent GC-MS analysis, showed the presence of C16-C30 fatty acids in the bound form, whereas methylation of native wax indicated the presence of C18-C30 fatty acids in the free form. Acetylation of hydrolysis product showed the presence of C18-C30 fatty alcohols in the bound form, where as acetylation of native wax indicated that the C18-C32 fatty alcohols were in the free form. n-Triacontanol (C30), a plant growth regulator, was found in both free and bound forms.

(PDF emailed within 0-6 h: $19.90)

Accession: 012844309

Download citation: RISBibTeXText

DOI: 10.1016/j.foodchem.2006.04.008

Related references

Fatty acids, fatty alcohols, wax esters, and methyl esters from Crambe abyssinica and Lunaria annua seed oils. J Amer Oil Chem Soc 40(12): 742-744, 1963

The use of durapak column packings for the gas chromatographic separation of methyl esters of short and long chain fatty acids free fatty acids and fatty aldehydes. Journal of Chromatographic Science 8(5): 279-281, 1970

Fatty acid ethyl esters synthesized from ethanol and fatty acids are much weaker mitochondrial uncouplers than free fatty acids. Gastroenterology 106(4 SUPPL ): A976, 1994

Fatty acids, fatty alcohols, and wax esters from Limnanthes douglasii seed oil. Jour Amer Oil Chem Soc : 320-322, 1962

Analysis of free fatty acids, fusel alcohols, and esters in beer: an alternative to CS2 extraction. Journal of the American Society of Brewing Chemistser 52(3): 127-134, 1994

Influence of sunflower diseases on the seed oil and on changes in free fatty acids. 1970

Contents of total and free fatty acids in commercial sunflower seed oil. Nauchni Trudove, Vissh Institut po Khranitelna i Vkusova Promyshlennost 18(1): 177-181, 1971

Effects of insulin on the hepatic output of free fatty acids and on the release of free fatty acids from the peripheral fatty tissue. Turk Tip Cemiyeti Mecmuasi 36(3): 155-163, 1970

Process for obtaining fatty alcohols from free fatty acids us patent 4804790 february 14 1989. Official Gazette of the United States Patent & Trademark Office Patents 1099(2): 876, 1989

Studies on acid oils and fatty acids for chickens. II. Effect of free fatty acid content and degree of saturation of free fatty acids and neutral fat on fatty acid digestibility. British Poultry Science 37(1): 119-130, 1996

The use of a computer in the determination by gas-liquid chromatography of the concentration and identification of individual fatty acids present as free fatty acids, triglycerides and cholesteryl esters. Journal of Chromatography 30(1): 62-76, 1967

Effect of free fatty acids on the hydrogenation rate of sunflower oil and fatty acids mixture. 1975

Use of copper chromite, catalysts for hydrogenation of fatty acids and fatty acid esters to fatty alcohols. Fett Wissenschaft Technologie: Organ der Deutschen Gesellschaft fur Fettwissenschaft eV = Fat science technology 89(13): 508-512, 1987

Biodiesel production from crude Jatropha curcas L. seed oil with a high content of free fatty acids. Bioresource Technology 99(6): 1716-1721, 2007