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Cloning and sequence analyzing of the centromeric repeated sequences in silver

Cloning and sequence analyzing of the centromeric repeated sequences in silver

Animal Biotechnology Bulletin 10(1): 598-601

The prominent 750-bp ethidium bromide stained bands were recovered by electrophoresis after restriction enzyme digestion by Sac I. The fragments were introduced into DH5a of Escherichia coli using the pUC18 vector. The recombinant plasmid was identified by PCR; 10 recombinant clones were sequenced. Sequence analysis revealed that 9 sequences had 80% homology to known dog satellite DNA sequence, and that the fragment was from 741 to 743 bp. The homology among the 9 sequences was from 90 to 97%.

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