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Monoxenic culture of the arbuscular mycorrhizal fungus Scutellospora calospora and Ri-TDNA transformed carrot roots



Monoxenic culture of the arbuscular mycorrhizal fungus Scutellospora calospora and Ri-TDNA transformed carrot roots



New Zealand Plant Protection 59: 97-102



This paper presents the first report of cultivation of Scutellospora calospora with Ri-TDNA transformed carrot (Daucus carota. L) roots. Carrot root transformation was done using Agrobacterium rhizogenes. Spore germination tests were conducted on 6% water agar and minimal media to assess the time taken for germination. Morphological characteristics of extraradical mycelium, intraradical mycelium, branched absorbing structures and auxiliary cell formation were recorded. Auxiliary cell formation started within 3-5 days of root contact and continued until 2 months. There was an extensive, thick, brown extraradical mycelial development following root infection, but spore formation was low (four spores) and was observed only after 8 months. Spores were full of oil globules and were produced on MSR medium but not M medium.

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Accession: 012873849

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