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Purification and biological effects of Araucaria angustifolia (Araucariaceae) seed lectin

Purification and biological effects of Araucaria angustifolia (Araucariaceae) seed lectin

Biochemical and Biophysical Research Communications 350(4): 1050-1055

This paper describes the purification and characterization of a new N-acetyl-d-glucosamine-specific lectin from Araucaria angustifolia (AaL) seeds (Araucariaceae) and its anti-inflammatory and antibacterial activities. AaL was purified using a combination of affinity chromatography on a chitin column and ion exchange chromatography on Sephacel-DEAE. The pure protein has 8.0kDa (SDS-PAGE) and specifically agglutinates rabbit erythrocytes, effect that was independent of the presence of divalent cations and was inhibited after incubation with glucose and N-acetyl-d-glucosamine. AaL showed antibacterial activity against Gram-negative and Gram-positive strains, shown by scanning electron microscopy. AaL, intravenously injected into rats, showed anti-inflammatory effect, via carbohydrate site interaction, in the models of paw edema and peritonitis. This lectin can be used as a tool for studying bacterial infections and inflammatory processes.

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Accession: 012882238

Download citation: RISBibTeXText

PMID: 17045568

DOI: 10.1016/j.bbrc.2006.09.149

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