+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Stimulation of eryptosis by aluminium ions



Stimulation of eryptosis by aluminium ions



Toxicology and Applied Pharmacology 217(2): 168-175



Aluminium salts are utilized to impede intestinal phosphate absorption in chronic renal failure. Toxic side effects include anemia, which could result from impaired formation or accelerated clearance of circulating erythrocytes. Erythrocytes may be cleared secondary to suicidal erythrocyte death or eryptosis, which is characterized by cell shrinkage and exposure of phosphatidylserine (PS) at the erythrocyte surface. As macrophages are equipped with PS receptors, they bind, engulf and degrade PS-exposing cells. The present experiments have been performed to explore whether Al(3+) ions trigger eryptosis. The PS exposure was estimated from annexin binding and cell volume from forward scatter in FACS analysis. Exposure to Al(3+) ions (> or =10 microM Al(3+) for 24 h) indeed significantly increased annexin binding, an effect paralleled by decrease of forward scatter at higher concentrations (> or =30 microM Al(3+)). According to Fluo3 fluorescence Al(3+) ions (> or =30 microM for 3 h) increased cytosolic Ca(2+) activity. Al(3+) ions (> or =10 microM for 24 h) further decreased cytosolic ATP concentrations. Energy depletion by removal of glucose similarly triggered annexin binding, an effect not further enhanced by Al(3+) ions. The eryptosis was paralleled by release of hemoglobin, pointing to loss of cell membrane integrity. In conclusion, Al(3+) ions decrease cytosolic ATP leading to activation of Ca(2+)-permeable cation channels, Ca(2+) entry, stimulation of cell membrane scrambling and cell shrinkage. Moreover, Al(3+) ions lead to loss of cellular hemoglobin, a feature of hemolysis. Both effects are expected to decrease the life span of circulating erythrocytes and presumably contribute to the development of anemia during Al(3+) intoxication.

(PDF emailed within 0-6 h: $19.90)

Accession: 012889240

Download citation: RISBibTeXText

PMID: 17055015

DOI: 10.1016/j.taap.2006.09.001


Related references

Stimulation of eryptosis by cadmium ions. Cellular Physiology and Biochemistry 22(1-4): 245-252, 2008

Oxidative stress due to aluminium exposure induces eryptosis which is prevented by erythropoietin. 2012

Phytotoxicity of monomer aluminium ions and hydroxy-aluminium polymer ions in an Andosol. Plant soil interactions at low pH: principles and management Proceedings of the Third International Symposium, Brisbane, Queensland, Australia, 12-16 September 1993: 367-370, 1995

Stimulation of Fe-2+-induced lipid peroxidation in phosphatidylcholine liposomes by aluminium ions at physiological pH. Biochimica et Biophysica Acta 1170(2): 182-188, 1993

Solubility of simple aluminium compounds expected in soils III Aluminium ions in soil solutions and aluminium phosphates in soils. Australian Journal of Soil Research 1(1): 46, 1963

Stimulation of Eryptosis by Afatinib. Cellular Physiology and Biochemistry 47(3): 1259-1273, 2018

Stimulation of eryptosis by cryptotanshinone. Cellular Physiology and Biochemistry 34(2): 432-442, 2015

Stimulation of Eryptosis by Narasin. Cellular Physiology and Biochemistry 37(5): 1807-1816, 2016

Stimulation of Eryptosis by Caspofungin. Cellular Physiology and Biochemistry 39(3): 939-949, 2016

Stimulation of eryptosis by anti-A IgG antibodies. Cellular Physiology and Biochemistry 20(5): 591-600, 2007

Exchange-adsorption characteristics of aluminium and manganous ions by red soils. IV. Chemical phenomenon for exchange of calcium/potassium ion by aluminium/manganous ions. Acta Pedologica Sinica 42(1): 64-69, 2005

Sulindac sulfide--induced stimulation of eryptosis. Cellular Physiology and Biochemistry 30(4): 1072-1082, 2013

Stimulation of Eryptosis, the Suicidal Erythrocyte Death by Piceatannol. Cellular Physiology and Biochemistry 38(6): 2300-2310, 2016

Stimulation of Eryptosis by Combretastatin A4 Phosphate Disodium (CA4P). Cellular Physiology and Biochemistry 38(3): 969-981, 2016

Adsorption of aluminium ions by montmorillonite: influence on aluminium speciation. Netherlands Journal of Agricultural Science 35(3): 259-269, 1987