+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of castor contamination by real-time polymerase chain reaction



Detection of castor contamination by real-time polymerase chain reaction



Journal of Agricultural and Food Chemistry 55(2): 545-550



Due to the potential for intentional contamination of food with crude preparations containing ricin, a real-time PCR method was developed for the detection of castor plant material in ground beef. One primer pair was identified and confirmed to be castor-specific and efficient for amplification of ricin in DNA extracts from castor or beef matrices. Of three different DNA extraction protocols compared, the hexadecyltrimethylammonium bromide (CTAB) method yielded the highest quality of DNA for QPCR assay. The detection limit for castor contamination in ground beef samples was <0.001% (<10 microg of castor acetone powder per gram of beef, corresponding to 0.5 microg of ricin), indicating excellent sensitivity for the assay, well below the threshold for oral toxicity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012908277

Download citation: RISBibTeXText

PMID: 17227091

DOI: 10.1021/jf062381r


Related references

Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood. Diagnostic Microbiology and Infectious Disease 66(1): 41-49, 2010

Application of a real time polymerase chain reaction method to detect castor toxin contamination in fluid milk and eggs. Journal of Agricultural and Food Chemistry 55(17): 6897-6902, 2007

Optimal sampling time after preparation of platelet concentrates for detection of bacterial contamination by quantitative real-time polymerase chain reaction. Vox Sanguinis 89(4): 208-214, 2005

Detection of breast cancer cell contamination in leukapheresis product by real-time quantitative polymerase chain reaction. Bone Marrow Transplantation 27(5): 517-523, 2001

Real-time polymerase chain reaction in transfusion medicine: applications for detection of bacterial contamination in blood products. Transfusion Medicine Reviews 21(3): 237-254, 2007

Evaluation of a commercial real-time polymerase chain reaction assay for detection of environmental contamination with Clostridium difficile. Journal of Hospital Infection 85(1): 76-78, 2013

Rapid detection of mycoplasma contamination in cell cultures using SYBR Green-based real-time polymerase chain reaction. In Vitro Cellular and Developmental Biology. Animal 42(3-4): 63-69, 2006

Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chain reaction. Food Additives and Contaminants. Part A, Chemistry, Analysis, Control, Exposure and Risk Assessment 26(2): 180-188, 2010

Rapid and accurate detection of Arcobacter contamination in commercial chicken products and wastewater samples by real-time polymerase chain reaction. Foodborne Pathogens and Disease 7(3): 327-338, 2010

Comparison of a conventional polymerase chain reaction with real-time polymerase chain reaction for the detection of neurotropic viruses in cerebrospinal fluid samples. Indian Journal of Medical Microbiology 29(2): 102-109, 2011

Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction with Parasitological Methods for Detection of Strongyloides stercoralis in Human Fecal Samples. American Journal of Tropical Medicine and Hygiene 93(6): 1285-1291, 2015

Development of an ethidium monoazide-enhanced internally controlled universal 16S rDNA real-time polymerase chain reaction assay for detection of bacterial contamination in platelet concentrates. Transfusion 52(7): 1423-1432, 2012

Specificity of a quantitative real-time polymerase chain reaction assay for the detection of invasive pneumococcal disease: identifying streptococcus pneumoniae using quantitative polymerase chain reaction. Chest 137(1): 243-244, 2010

Comparison of two real-time quantitative polymerase chain reaction strategies for minimal residual disease evaluation in lymphoproliferative disorders: correlation between immunoglobulin gene mutation load and real-time quantitative polymerase chain reaction performance. Hematological Oncology 32(3): 133-138, 2014

Immunopolymerase chain reaction using real-time polymerase chain reaction for detection. Analytical Biochemistry 281(2): 230-232, 2000