Mutagenic efficiency of gamma-rays, ethyl methane sulphonate and its combination on microsperma lentil (Lens culinaris Medik)

Singh, S.P.; Singh, R.P.; Singh, N.K.; Prasad, J.P.; Shahi, J.P.

International Journal of Agricultural Sciences 3(1): 113-118


Accession: 012922316

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Seeds of microsperma lentil (Lens culinaris) cv. Pant L-406 were selected for mutagenic treatment. Fresh healthy seeds were irradiated with 10, 15, 20, 25 and 30kR doses of gamma-rays. Healthy seeds were also pre-soaked in distilled water and treated with different concentrations of ethyl methanesulfonate (EMS) (0.02, 0.03, 0.04 and 0.05 M). Half of the irradiated seeds were also treated with 0.03 M of EMS for combination treatments. Observations were recorded for percent germination, seedling height, pollen fertility, plant survival and chromosomal abnormalities in M generation. Frequency and spectrum of chlorophyll and viable mutations were scored in the M generation. Higher doses of mutagen either alone and/or in combination severely reduced the percentage of germination, seedling height, pollen fertility and plant survival than their corresponding lower doses. The percent chromosomal abnormalities, i.e. fragments, bridges, laggards and micronuclei, increased with increasing dosage/concentration of mutagens. Mutagenic efficiency was computed on the basis of seedling injury, lethality and pollen sterility. Lower dose of EMS (0.02 M) was found to be most efficient in inducing more chlorophyll and viable mutations. Various types of chlorophyll and viable mutations were identified from all the treatments.