+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Application of pressure-modulated differential scanning calorimetry to the determination of relaxation kinetics of multilamellar lipid vesicles



Application of pressure-modulated differential scanning calorimetry to the determination of relaxation kinetics of multilamellar lipid vesicles



Biophysical Chemistry 126(1-3): 218-227



We report an extension of the recently published PMDSC method that permitted synchronous determination of heat capacity and expansibility when using slow, defined pressure formats in a DSC scan. Here we applied continuously opposing pressure changes that are fast compared to the time constants of the DSC instrument to study relaxation kinetics of phospholipids. Investigations of multilamellar vesicles of DPPC or DSPC in water revealed for both lipids relaxation times of about 30 s at the maximum of the main transition peak and about 15 s at the maximum of the pretransition. The relaxation times in the transition range are proportional to heat capacity of main- and pretransition. The molecular origin of the relaxation processes appears to stem from pressure-induced water fluxes between the interbilayer region and the bulk water phase.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012945760

Download citation: RISBibTeXText

PMID: 16831505

DOI: 10.1016/j.bpc.2006.06.004


Related references

Differential scanning calorimetry of multilamellar vesicles of dipalmitoyl phosphatidylcholine in solutions of trehalose. Cryobiology 27(6): 683, 1990

Pressure-modulated differential scanning calorimetry. An approach to the continuous, simultaneous determination of heat capacities and expansion coefficients. Analytical Chemistry 78(4): 984-990, 2006

Effect of penetration enhancers (pyrrolidone derivatives) on multilamellar liposomes of stratum corneum lipid: A study by UV spectroscopy and differential scanning calorimetry. International Journal of Pharmaceutics (Amsterdam) 95(1-3): 43-50, 1993

Enthalpy relaxation behavior of dry wood detected by temperature-modulated differential scanning calorimetry. Journal of Wood Science 58(5): 391-398, 2012

Characterisation of the relaxation behaviour of freeze dried lactose using temperature-modulated differential scanning calorimetry. Journal of Pharmacy & Pharmacology 51(SUPPL ): 210, Sept, 1999

Pressure-modulated differential scanning calorimetry: theoretical background. Analytical Chemistry 78(4): 991-996, 2006

Determination of physical changes of inulin related to sorption isotherms: an X-ray diffraction, modulated differential scanning calorimetry and environmental scanning electron microscopy study. Carbohydrate Polymers 63(2): 210-217, 2006

Determination of amylose content in different starches using Modulated Differential Scanning Calorimetry. Starch/Starke 58(5): 209-214, 2006

Freeze/thaw effects on lipid-bilayer vesicles investigated by differential scanning calorimetry. Biochimica et Biophysica Acta 1615(1-2): 77-83, 2 September, 2003

Modeling leakage kinetics from multilamellar vesicles for membrane permeability determination: application to glucose. Biophysical Journal 91(12): 4340-4349, 2006

The coupling of relaxation times to the heat capacity of lipid vesicles studied by pressure jump calorimetry. Biophysical Journal 80(1 Part 2): 502a, January, 2001

Kinetics of rapeseed oil oxidation by pressure differential scanning calorimetry measurements. European Journal of Lipid Science & Technology 102(5): 337-341, 2000

Application of TZERO calibrated modulated temperature differential scanning calorimetry to characterize model protein formulations. International Journal of Pharmaceutics 309(1-2): 146-156, 2006

Effect of the hydrophobic peptide t is from mn glyco protein on sodium efflux and differential scanning calorimetry of phospho lipid bi layer vesicles. Biophysical Journal 17(2): 70A, 1977

Investigation of the starch gelatinisation phenomena in water-glycerol systems: application of modulated temperature differential scanning calorimetry. Carbohydrate Polymers 58(2): 191-204, 2004