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Chapter 12,961

Development of a liquid chromatography/electrospray ionization tandem mass spectrometric method for the determination of hydroxyl radical

Yang, F.; Zhang, R.; He, J.; Abliz, Z.

Rapid Communications in Mass Spectrometry: Rcm 21(2): 107-111

2007


ISSN/ISBN: 0951-4198
PMID: 17154348
DOI: 10.1002/rcm.2815
Accession: 012960884

A rapid method combining liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was developed for the determination of the hydroxyl radical (.OH). .OH generated via Fenton reaction was spin-trapped by 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and then analyzed by LC/ESI-MS/MS in multiple reaction monitoring (MRM) mode, using N-methyl-2-pyrrolidone (NMP) as the internal standard. The peak area ratio of DMPO-OH to NMP positively correlated with the concentration of .OH. The relative standard deviation (RSD) of the method was 1.13% (n = 8). The present method was successfully applied to evaluate the .OH scavenging capacity of several phenolic acids.

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