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Quantitative recovery of Listeria monocytogenes and select salmonella serotypes from environmental sample media

, : Quantitative recovery of Listeria monocytogenes and select salmonella serotypes from environmental sample media. Journal of Aoac International 90(1): 250-257

Environmental sampling has become increasingly important in the food industry for monitoring the presence of specific pathogens such as Listeria monocytogenes and Salmonella enterica. Several microbiological media are available for storage and transport of environmental samples from the processing plant to the test laboratory. In this study, we quantified the survival of L. monocytogenes, S. Typhimurium, S. Enteritidis, and S. Typhi in environmental sampling media over several time and temperature combinations to determine optimum conditions for transport and storage. A cocktail of L. monocytogenes strains and Salmonella serotypes was separately added to tubes of Dey-Engley (D/E) Neutralizing Broth, Copan SRK solution, and Neutralizing Buffer and incubated at either -4, 4, 10, or 15 degree C. Counts were made of the bacterial load after 0, 12, 24, and 48 h. Neutralizing Buffer and Copan SRK solution were best at maintaining bacterial concentrations at all temperatures. D/E Neutralizing Broth, at 10 and 15 degrees C, allowed significant bacterial growth. This study helped validate the use of these 3 media for environmental sample transport and storage at cold holding temperatures and demonstrated that, at elevated temperatures (>4 degrees C), it is preferable to use Neutralizing Buffer or Copan SRK solution for quantifying microbial recovery.

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Accession: 013007972

PMID: 17373457

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Espeseth, D.A., 1995: Present systems and future needs for risk assessment of biologicals in the United States of America: the perspective of the regulator. The authority for regulating veterinary biologicals in the United States of America (USA) is provided in the Virus-Serum-Toxin Act, enacted in 1913 and amended in 1995. The Act authorizes the Secretary of Agriculture to prescribe regulations gover...

Jarvis, R.R.; Jebakumar, S.; Bhattacharyya, M.N.; Mackway-Jones, K., 1998: Emergency unit care of sexually transmitted infections. The aim of the study was to assess provisions for management of sexually transmitted infections (STIs), emergency contraception and pregnancy test in UK emergency departments. Postal questionnaires were sent to all consultant-led emergency departm...

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Ganzer U.; Nitze H.R., 1972: The dna content of various benign and malignant tumors. Archiv fuer Klinische und Experimentelle Ohren- Nasen- und Kehlkopfheilkunde 199(2): 629-634, 1971

Melin, B.; Bittel, J.; Picard, J.; Pibarot, A.; Bigard, X.; Brion, R.; Guezennec, C. Y., 1995: Assessment of physical fitness by questionnaire. A questionnaire which examined medical antecedents and physical activities was tested on 505 young males recently incorporated in army. A linear model was determined using indices for biometry and sport activities and was satisfying to estimate ae...

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Baca A.; S. F., 1961: Fattening sheep at high altitude. 1. Urea and antibiotics combined with implants of diethylstilboestrol and of progesterone and oestradiol benzoate, and vitamin A for fattening wethers.. Merino X Rambouillet sheep 9 to 10 months of age in 5 groups of 48 on pasture at 3800 metres above sea level in Peru were given no supplement or mixtures with cottonseed meal as the main source of protein or with urea to supply 30% of the digestib...

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Reichert H.; Wine J.J., 1983: Coordination of lateral giant and nongiant systems in crayfish procambarus clarkii escape behavior. The modal escape response of a well-rested crayfish to a tap on the abdomen consists of an initial lateral giant-mediated flexion, a movement-triggered reflex extension and then a series of centrally patterned, non-giant mediated, extension-flexio...

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