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ROS mediates baicalin-induced apoptosis in human promyelocytic leukemia HL-60 cells through the expression of the Gadd153 and mitochondrial-dependent pathway



ROS mediates baicalin-induced apoptosis in human promyelocytic leukemia HL-60 cells through the expression of the Gadd153 and mitochondrial-dependent pathway



Anticancer Research 27(1a): 117-125



Background: Chemotherapy agents, particularly those that can induce apoptosis', (ire the major intervening strategy in the treatment of leukemia. In this study, we investigated the effects of baicalin (a compound obtained from Sculellaria baicalensis Georgi and S. rivularis Benth Labiateae) on the viability, induction of apoptosis and associated mechanism in human leukemia HL-60 cells. Materials and Methods: The cell viability and apoptosis was examined by flow cytometric analysis. The results showed that baicalin induced cytotoxicity in a dose- and time-dependent manner through the activation of caspase-3., as shown by treatment of HL-60 cells with (in inhibitor of caspase-3 (z-VAD-fink). Baicalin increased the levels of ROS, Ca2+ and decreased mitochondrial membrane potential in HL-60 cells. Western blot demonstrated that baicalin promoted the levels of Gadd531, Bax, cytochrome c and caspase-3 and -12, but decreased the levels of Gip78 and Bcl-2 in HL-60 cells. Conclusion: Baicalin was found to induce apoptosis in HL-60 cells through. multiple pathways.

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Accession: 013008184

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PMID: 17352223


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