+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

T-cell receptor repertoire usage after allografting differs between CD4+CD25+ regulatory T cells and their CD4+CD25- counterpart

T-cell receptor repertoire usage after allografting differs between CD4+CD25+ regulatory T cells and their CD4+CD25- counterpart

Haematologica 92(2): 206-214

Background and Objectives After allogeneic haematopoietic stem cell transplantation (SCT) the whole T-cell receptor (TCR) repertoire shows a markedly skewed pattern for 2-3 years. A small fraction of CD4(+) T cells is represented by CD25(+) regulatory lymphocytes (T, g), which play a crucial role in modulating peripheral tolerance. To investigate their ability to react to the massive antigenic stimulation generated in an allogeneic host, which could significantly affect their pattern of reconstitution, we analyzed the TCR repertoire of T-reg after SCT, focusing on the degree of similarity to CD4(+)CD25(-) conventional T cells (T-conv).Design and Methods We assessed the TCR V beta repertoire of T-reg in ten patients who had received allogeneic SCT, by using complementarity determining region 3 (CDR3) spectratyping. We developed a new similarity score for the analysis. This score expresses the proportion of V beta with similar profile between T-reg and T-conv.Results For up to 3 years after SCT the repertoires of Treg and Tconv were characterized by several V beta with different profiles between the two cell subsets, while they were extremely similar in patients more than 3 years post-allografting (similarity score=0.90 vs. 0.61). The differences observed early after SCT were mainly ascribable to V beta expressing an oligoclonal profile in T-(conv) but not in T-reg.Interpretation and Conclusions Our data show that the TCR repertoires of Treg and Tconv are significantly different early post-SCT, while they tend to become identical with full reconstitution. This difference could reflect either a discrepancy in the in vivo reactivity against common antigenic stimulations or be the result of different post-transplant ontogeny.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 013020600

Download citation: RISBibTeXText

PMID: 17296570

DOI: 10.3324/haematol.10774

Related references

T cell receptor beta chain (TCR-Vbeta) repertoire of circulating CD4(+) CD25(-), CD4(+) CD25(low) and CD4(+) CD25(high) T cells in patients with long-term renal allograft survival. Transplant International 23(1): 54-63, 2010

Cutting edge: Size and diversity of CD4(+) CD25(high) Foxp3(+) regulatory T cell repertoire in humans: evidence for similarities and partial overlapping with CD4(+) CD25(-)T cells. Journal of Immunology 179(6): 3412-3416, 2007

Follicular lymphoma intratumoral CD4+CD25+GITR+ regulatory T cells potently suppress CD3/CD28-costimulated autologous and allogeneic CD8+CD25- and CD4+CD25- T cells. Journal of Immunology 178(7): 4051-4061, 2007

Partial CD25 Antagonism Enables Dominance of Antigen-Inducible CD25 high FOXP3 + Regulatory T Cells As a Basis for a Regulatory T Cell-Based Adoptive Immunotherapy. Frontiers in Immunology 8: 1782, 2018

The Il-4 Receptor -Chain-Binding Cytokines, Il-4 and Il-13, Induce Forkhead Box P3-Expressing Cd25+Cd4+ Regulatory T Cells from Cd25-Cd4+ Precursors. The Journal of Immunology 175(9): 6107-6116, 2005

The IL-4 Receptor a-Chain-Binding Cytokines, IL-4 and IL-13, Induce Forkhead Box P3-Expressing CD25+CD4+ Regulatory T Cells from CD25-CD4+ Precursors. Journal of Immunology 175(9): 07-16, 2005

The IL-4 receptor alpha-chain-binding cytokines, IL-4 and IL-13, induce forkhead box P3-expressing CD25+CD4+ regulatory T cells from CD25-CD4+ precursors. Journal of Immunology 175(9): 6107-6116, 2005

MicroRNAs are implicated in the suppression of CD4+CD25− conventional T cell proliferation by CD4+CD25+ regulatory T cells. Molecular Immunology 63(2): 464-472, 2015

Cutting edge: IL-4-induced protection of CD4+CD25- Th cells from CD4+CD25+ regulatory T cell-mediated suppression. Journal of Immunology 176(7): 3900-3904, 2006

Regulation of murine inflammatory bowel disease by CD25+ and CD25- CD4+ glucocorticoid-induced TNF receptor family-related gene+ regulatory T cells. Journal of Immunology 171(2): 708-716, 2003

Control of T cell hyperactivation in IL-2-deficient mice by CD4+CD25- and CD4+CD25+ T cells: Evidence for two distinct regulatory mechanisms. European Journal of Immunology 31(6): 1637-1645, 2001

Antigen-specific T cell repertoire modification of CD4+CD25+ regulatory T cells. Journal of Immunology 172(9): 5240-5248, 2004

Conversion of peripheral CD4(+) CD25(-) naive T cells to CD4(+) CD25(+) regulatory T cells by ovarian carcinoma cell supernatants. Zhonghua Weishengwuxue He Mianyixue Zazhi 26(2): 121-124, 2006

Effect of CD4+CD25+ and CD4+CD25- T regulatory cells on the generation of cytolytic T cell response to a self but human tumor-associated epitope in vitro. Journal of Immunology 176(2): 984-990, 2006

MRL/Mp CD4+,CD25- T cells show reduced sensitivity to suppression by CD4+,CD25+ regulatory T cells in vitro: a novel defect of T cell regulation in systemic lupus erythematosus. Arthritis and Rheumatism 52(4): 1180-1184, 2005