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Blue light but not red light induces a calcium transient in the moss Physcomitrella patens (Hedw.) B., S. & G



Blue light but not red light induces a calcium transient in the moss Physcomitrella patens (Hedw.) B., S. & G



Planta 206(2): 278-283



In caulonemal filaments of the moss, Physcomitrella patens, which had been incubated in darkness, 3 s irradiation with blue light (lambda(max) 450 nm) at fluence rates of 100 micromole m-2 s-1 and above caused a transient increase in cytosolic calcium ion concentration. [Ca2+]cyt, which was both intensity- and time-dependent. Measurements of [Ca2+]cyt were made using moss transformed with the cDNA for apoaequorin and reconstituting the Ca2+ -dependent photoprotein aequorin in the cytosol by incubation in coelenterazine. In response to blue light at fluence rates of 100-1000 micromole photons m-2 s-1, [Ca2+]cyt increased transiently from a basal level of approximately 50 nM to between 200 and 700 nM. Irradiation with red light did not evoke any measurable change in [Ca2+]cyt. The presence of calcium in the incubating medium was not required for the increase in [Ca2+]cyt to occur. A mutant strain, gad-139, was identified which required an irradiance of only 1 s to evoke a response. The kinetics showed a delay of approximately 6 s from the beginning of illumination before the beginning of the increase in [Ca2+]cyt. The data suggest that the activation of a photoreceptor rather than the direct opening of calcium channels is involved in this blue-light response.

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Accession: 013087141

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DOI: 10.1007/s004250050401


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