+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Competition ELISA, using monoclonal antibodies to the transmissible gastroenteritis antibodies to the transmissible gastroenteritis virus (TGEV) S protein, for serologic differentiation of pigs infected with TGEV or porcine respiratory coronavirus



Competition ELISA, using monoclonal antibodies to the transmissible gastroenteritis antibodies to the transmissible gastroenteritis virus (TGEV) S protein, for serologic differentiation of pigs infected with TGEV or porcine respiratory coronavirus



American journal of veterinary research 54(2): 254-259



Monoclonal antibodies (MAB) to subsite A (25C9) and subsite D (44C11) of the S protein of transmissible gastroenteritis virus (TGEV) were used in a blocking ELISA on fixed TGEV-infected swine testis cells to differentiate sera from pigs experimentally inoculated with either TGEV or porcine respiratory coronavirus (PRCV). Serum samples were obtained from pigs at various intervals from postinoculation day (PID) 0 through at least PID 22 to 40. Eleven-day-old pigs, seronegative for TGEV-neutralizing antibodies at the time of inoculation, were inoculated orally and nasally with either the virulent Miller (M5C) strain or the attenuated Purdue (P115) strain of TGEV, or with the ISU-1 strain of PRCV. Gastroenteritis was observed in 100% of the M5C-TGEV-inoculated pigs; but clinical signs of disease were not observed in either the P115-TGEV- or PRCV-inoculated pigs. Virus-neutralization (VN) antibody titer in sera was determined by use of a plaque-reduction assay. Blocking ELISA antibody titer for subsites A and D was determined from the serum dilution that produced 50% reduction in the absorbance values when it competed with biotinylated MAB 25C9 and 44C11, respectively. In sera from the inoculated pigs, the VN antibody titer began to increase by PID 7 and reached maximum by PID 15 to 16. For pigs inoculated with TGEV M5C, subsite A and subsite D blocking antibody titers in the serum paralleled the VN antibody titer, began to increase after PID 7, and reached maximum by PID 15 to 16. The blocking antibody titer to subsites A and D began to increase in the P115-TGEV-inoculated pigs after PID 15 to 16 and reached maximum by PID 22 to 26. Blocking antibody titer to subsite A in PRCV-inoculated pigs behaved similarly to blocking antibody titer to subsite A in the M5C-TGEV-inoculated pigs, reaching maximum by PID 15 to 16; however, blocking antibody titer was not detected for subsite D up to PID 24 (the latest time point examined) in sera from the PRCV-inoculated pigs. Serum antibody responses and clinical signs of disease were monitored in pigs initially inoculated with either M5C-TGEV or -PRCV and challenge-exposed with M5C-TGEV on PID 24. Clinical signs of gastroenteritis were not observed in the M5C-TGEV-inoculated pigs after challenge-exposure with M5C-TGEV. Low increases in VN antibody titer and in subsite A or D blocking antibody titer were detected in the M5C-TGEV-inoculated and challenge-exposed pigs. Of the 12 pigs initially inoculated with PRCV then challenge-exposed with M5C-TGEV, 5 pigs developed diarrhea; the VN and subsite A antibody blocking titers began to increase by postchallenge-exposure day (PCD) 2 and reached maximal titer by PCD 9, increasing approximately 100-fold above the prechallenge-exposure titer. Subsite D antibody-blocking titer began to appear after PCD 9 and, by PCD 12, had reached nearly the same level as that for the primary response to the M5C-TGEV inoculation.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 013098551

Download citation: RISBibTeXText


Related references

A competitive inhibition ELISA for the differentiation of serum antibodies from pigs infected with transmissible gastroenteritis virus (TGEV) or with the TGEV-related porcine respiratory coronavirus. Veterinary Microbiology 20(1): 9-19, 1989

Evaluation of the baculovirus-expressed S glycoprotein of transmissible gastroenteritis virus (TGEV) as antigen in a competition ELISA to differentiate porcine respiratory coronavirus from TGEV antibodies in pigs. Journal of Veterinary Diagnostic Investigation 11(3): 205-214, 1999

Antigenic variation among transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus strains detected with monoclonal antibodies to the S protein of TGEV. American Journal of Veterinary Research 53(7): 1253-1258, 1992

Field validation of a commercial blocking ELISA to differentiate antibody to transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus and to identify TGEV-infected swine herds. Journal of Veterinary Diagnostic Investigation 14(2): 97-105, 2002

Induction of antibodies protecting against transmissible gastroenteritis coronavirus (TGEV) by recombinant adenovirus expressing TGEV spike protein. Virology 213(2): 503-516, 1995

Infection with a new porcine respiratory coronavirus in Denmark: serologic differentiation from transmissible gastroenteritis virus using monoclonal antibodies. Advances in Experimental Medicine and Biology 276: 435-439, 1990

Immune response of sows vaccinated with attenuated transmissible gastroenteritis virus (TGEV) and recombinant TGEV spike protein vaccines and protection of their suckling pigs against virulent TGEV challenge exposure. American Journal of Veterinary Research 59(8): 1002-1008, 1998

Partial passive protection with two monoclonal antibodies and frequency of feeding of hyperimmune anti-transmissible gastroenteritis virus (TGEV) serum for protection of three-day-old piglets from a TGEV challenge infection. Journal of Veterinary Diagnostic Investigation 13(4): 290-296, 2001

Antibody response to transmissible gastroenteritis virus and/or porcine respiratory coronavirus in TGEV-immune or PRCV-immune pigs. Veterinary Immunology & Immunopathology 35(SUPPL ): 101-102, 1993

Molecular aspects of the relationship of transmissible gastroenteritis virus (TGEV) with porcine respiratory coronavirus (PRCV). Advances in Experimental Medicine and Biology 276: 441-446, 1990

The Viral Nucleocapsid Protein of Transmissible Gastroenteritis Coronavirus (Tgev) Is Cleaved by Caspase-6 and -7 during Tgev-Induced Apoptosis. Journal of Virology 74(9): 3975-3983, 2000

The viral nucleocapsid protein of transmissible gastroenteritis coronavirus (TGEV) is cleaved by caspase-6 and -7 during TGEV-induced apoptosis. Journal of Virology 74(9): 3975-3983, 2001

Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies. Virus Research 46(1-2): 111-124, 1996

Molecular characterization and pathogenesis of transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCV) field isolates co-circulating in a swine herd. Archives of Virology 145(6): 1133-1147, 2000

An ELISA for the detection of serum antibodies to both transmissible gastroenteritis virus and porcine respiratory coronavirus. British Veterinary Journal 147(4): 370-372, 1991