+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus IBV-induced membrane fusion occurs at near-central pH

Coronavirus IBV-induced membrane fusion occurs at near-central pH

Archives of virology22(3-4): 307-316

The lysosomotropic agent NH4Cl caused a reduction of 80-95% in the number of chick kidney (CK) cells and Vero cells infected by infectious bronchitis virus (IBV) strain Beaudette, as determined by immunofluorescence at the end of the first replication cycle. Inhibition only occurred when NH4Cl was present during the first 2 h after infection. Syncytium formation was studied during replication of IBV-Beaudette in Vero cells. Some cell-cell fusion occurred at pH 7.0 and pH 6.5 but it was optimal at pH 6.7. IBV strain UK/123/82 did not replicate in Vero cells and was studied in CK cells in which it grew well but without forming syncytia. In contrast to IBV-Beaudette, NH4Cl had virtually no effect on the replication of UK/123/82. The results show that the IBV spike glycoprotein induces membrane fusion at near neutral pH although some IBV strains may require a mildly acidic environment for the efficient uncoating of the virion RNA.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 013101368

Download citation: RISBibTeXText

PMID: 1309994

Related references

Coronavirus-induced membrane fusion requires the cysteine-rich domain in the spike protein. Virology 269(1): 212-224, 2000

Characterization of HCoV-229E fusion core: implications for structure basis of coronavirus membrane fusion. Biochemical and Biophysical Research Communications 345(3): 1108-1115, 2006

Structural basis for coronavirus-mediated membrane fusion. Crystal structure of mouse hepatitis virus spike protein fusion core. Journal of Biological Chemistry 279(29): 30514-30522, 2004

Coronavirus-induced demyelination occurs in the absence of CD28 costimulatory signals. Journal of Neuroimmunology 146(1-2): 140-143, 2004

Identification of the membrane-active regions of the severe acute respiratory syndrome coronavirus spike membrane glycoprotein using a 16/18-mer peptide scan: implications for the viral fusion mechanism. Journal of Virology 79(3): 1743-1752, 2005

PH-dependent vesicle fusion induced by the ectodomain of the human immunodeficiency virus membrane fusion protein gp41: Two kinetically distinct processes and fully-membrane-associated gp41 with predominant β sheet fusion peptide conformation. Biochimica et Biophysica Acta 1848(1 Pt B): 289-298, 2015

Coronavirus-induced demyelination occurs in the absence of inducible nitric oxide synthase. Journal of Virology 74(16): 7683-7686, 2000

Coronavirus-induced demyelination occurs in the presence of virus-specific cytotoxic T cells. Virology 200(2): 733-743, 1994

Mechanism of protein-induced membrane fusion: fusion of phospholipid vesicles by clathrin associated with its membrane binding and conformational change. Biochemistry 28(3): 1422-1428, 1989

Modulation of membrane fusion by membrane fluidity temperature dependence of divalent cation induced fusion of phosphatidylserine vesicles. Biochemistry 24(1): 8-14, 1985

Single particle tracking assay to study coronavirus membrane fusion. Methods in Molecular Biology 1282: 183-194, 2015

Effect of mutations in a coronavirus surface glycoprotein on the ph dependence of membrane fusion. Journal of Cell Biology 111(5 Part 2): 320A, 1990

Mechanism of protein induced membrane fusion fusion of phospholipid vesicles by clathrin associated with membrane binding and conformational changes. Biochemistry 28(3): 1422-1428, 1989

The role of calcium and acidic phospho lipids in membrane fusion a contact induced local phase transition as a key event in membrane fusion phenomena. Federation Proceedings 39(6): ABSTRACT 2731, 1980