+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Immunological correlates for protection against intranasal challenge of Bacillus anthracis spores conferred by a protective antigen-based vaccine in rabbits



Immunological correlates for protection against intranasal challenge of Bacillus anthracis spores conferred by a protective antigen-based vaccine in rabbits



Infection and Immunity 74(1): 394-398



Correlates between immunological parameters and protection against Bacillus anthracis infection in animals vaccinated with protective antigen (PA)-based vaccines could provide surrogate markers to evaluate the putative protective efficiency of immunization in humans. In previous studies we demonstrated that neutralizing antibody levels serve as correlates for protection in guinea pigs (S. Reuveny et al., Infect. Immun. 69:2888-2893, 2001; H. Marcus et al., Infect. Immun. 72:3471-3477, 2004). In this study we evaluated similar correlates for protection by active and passive immunization of New Zealand White rabbits. Full immunization and partial immunization were achieved by single and multiple injections of standard and diluted doses of a PA-based vaccine. Passive immunization was carried out by injection of immune sera from rabbits vaccinated with PA-based vaccine prior to challenge with B. anthracis spores. Immunized rabbits were challenged by intranasal spore instillation with one of two virulent strains (strains Vollum and ATCC 6605). The immune competence was estimated by measuring the level of total anti-PA antibodies, the neutralizing antibody titers, and the conferred protective immunity. The results indicate that total anti-PA antibody titers greater than 1 x 10(5) conferred protection, whereas lower titers (between 10(4) and 10(5)) provided partial protection but failed to predict protection. Neutralizing antibody titers between 500 and 800 provided partial protection, while titers higher than 1,000 conferred protection. In conclusion, this study emphasizes that regardless of the immunization regimen or the time of challenge, neutralizing antibody titers are better predictors of protection than total anti-PA titers.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 013140351

Download citation: RISBibTeXText

PMID: 16368995

DOI: 10.1128/iai.74.1.394-398.2006


Related references

A plant-produced protective antigen vaccine confers protection in rabbits against a lethal aerosolized challenge with Bacillus anthracis Ames spores. Human Vaccines and Immunotherapeutics 9(3): 544-552, 2013

Contribution of immunological memory to protective immunity conferred by a Bacillus anthracis protective antigen-based vaccine. Infection and Immunity 72(6): 3471-3477, 2004

Correlation between lethal toxin-neutralizing antibody titers and protection from intranasal challenge with Bacillus anthracis Ames strain spores in mice after transcutaneous immunization with recombinant anthrax protective antigen. Infection and Immunity 74(1): 794-797, 2006

Correlation between lethal toxin neutralizing antibody titers and protection from intranasal challenge with Bacillus anthracis ames strain spores in mice afteir skin immunization with recombinaint anthrax protective antigen. 2005

An intranasal vaccine targeting both the Bacillus anthracis toxin and bacterium provides protection against aerosol spore challenge in rabbits. Vaccine 24(18): 3953-3963, 2006

Oral administration of a Salmonella enterica-based vaccine expressing Bacillus anthracis protective antigen confers protection against aerosolized B-anthracis. Infection and Immunity 75(4): 1827-1834, 2007

Oral administration of a Salmonella enterica-based vaccine expressing Bacillus anthracis protective antigen confers protection against aerosolized B. anthracis. Infection and Immunity 75(4): 1827-1834, 2007

Duration of protection of rabbits after vaccination with Bacillus anthracis recombinant protective antigen vaccine. Vaccine 24(14): 2530-2536, 2006

Comparative vaccine efficacy of different isoforms of recombinant protective antigen against Bacillus anthracis spore challenge in rabbits. Vaccine 24(17): 3469-3476, 2006

Anthrax vaccine antigen-adjuvant formulations completely protect New Zealand white rabbits against challenge with Bacillus anthracis Ames strain spores. Clinical and Vaccine Immunology 19(1): 11-16, 2012

Mucosal immunization with a novel nanoemulsion-based recombinant anthrax protective antigen vaccine protects against Bacillus anthracis spore challenge. Infection and Immunity 75(8): 4020-4029, 2007

Protective efficacy of a recombinant protective antigen against Bacillus anthracis challenge and assessment of immunological markers. Vaccine 16(8): 810-817, 1998

Intranasal immunization with protective antigen of Bacillus anthracis induces a long-term immunological memory response. Molecular Immunology 67(2 Pt B): 492-500, 2015

Recombinant GroEL enhances protective antigen-mediated protection against Bacillus anthracis spore challenge. Medical Microbiology and Immunology 202(2): 153-165, 2013

Immunological studies of anthrax. IL Levels of immunity against Bacillus anthracis obtained with protective antigen and live vaccine. J. Immunol, 88: 15, 1962