+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

In vivo stage- and tissue-specific DNA-protein interactions at the D. melanogaster alcohol dehydrogenase distal promoter and adult enhancer



In vivo stage- and tissue-specific DNA-protein interactions at the D. melanogaster alcohol dehydrogenase distal promoter and adult enhancer



Nucleic Acids Research 20(20): 5413-5422



We performed a high resolution analysis of the chromatin structure within the regions required for distal transcription of the Drosophila melanogaster alcohol dehydrogenase gene (Adh). Using dimethyl sulfate, DNase I, and micrococcal nuclease as structural probes, and comparing chromatin structure in tissues isolated from several developmental stages, we have identified several sites of stage- and tissue-specific DNA-protein interactions that correlate with distal transcription initiation. Most were within previously identified cis-acting elements and/or in vitro protein binding sites of the adult enhancer (AAE) and distal promoter, including the TATA box. We also detected a novel stage-specific DNA-protein interaction at the Adf-2a binding site where a non-histone protein was bound to the DNA on the surface of a positioned nucleosome previously identified between the distal promoter and adult enhancer. In addition to footprints, we have also revealed stage- and tissue-specific DNA helix deformations between many of the non-histone protein binding sites. These helix distortions suggest there are interactions among the adjacently bound proteins that result in bending or kinking of the intervening DNA. The distal promoter and AAE have an accessible chromatin conformation in fat body prior to the third larval instar and many of the regulatory proteins that bind in these regions are also available before distal transcription begins. Nevertheless, the timing of DNA-protein interactions in the distal promoter and AAE suggest these proteins do not bind individually or assemble progressively as they and their binding sites become available. Instead, there appears to be a coordinated assembly of a large cooperative complex of proteins interacting with the distal promoter, the positioned nucleosome, the enhancer of the distal promoter (the AAE), and each other.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 013142026

Download citation: RISBibTeXText

PMID: 1437559


Related references

In vivo stage- and tissue-specific Dna-protein interactions at the D.melanogaster alcohol dehydrogenase distal promoter and adult enhancer. Nucleic Acids Research 20(20): 5413-5422, 1992

The adult enhancer factor-1, a Drosophila melanogaster transcriptional repressor, modulates the promoter activity of the rat class-I alcohol dehydrogenase-encoding gene. Gene (Amsterdam) 149(2): 325-330, 1994

The binding site of a steroid hormone receptor-like protein within the Drosophila Adh adult enhancer is required for high levels of tissue-specific alcohol dehydrogenase expression. Molecular and Cellular Biology 12(2): 661-673, 1992

Complex organization of promoter and enhancer elements regulate the tissue- and developmental stage-specific expression of the Drosophila melanogaster Gld gene. Genetics 157(2): 699-716, 2001

The adult enhancer factor-1 , a Drosophila transcriptional repressor protein, modulates activities of the rat class I alcohol dehydrogenase promoter. Hepatology 18(4 Part 2): 123A, 1993

Interactions between distal enhancer and separate and distinct tissue specific promoter elements determine apo-lipoprotein AI gene expression in liver and intestine. Journal of Cellular Biochemistry Suppl. 0(17 Part A): 73, 1993

Developmental and environmental regulation of the maize alcohol dehydrogenase 1 (Adh1) gene: promoter-enhancer interactions. Plant gene systems and their biology Proceedings of a CIBA Geigy UCLA symposium held at Tamarron, Colorado, USA, 2-8 February 1987: 263-277, 1987

Dna - histone interactions are sufficient to position single nucleosome juxtaposing Drosophila Adh adult enhancer and distal promoter. Nucleic Acids Research 21(4): 957-967, 1993

DNA-histone interactions are sufficient to position a single nucleosome juxtaposing Drosophila Adh adult enhancer and distal promoter. Nucleic Acids Research 21(4): 957-967, 1993

In vivo and in vitro tissue specific interactions of a beta phaseolin enhancer like element with embryonic and vegetative nuclear protein factors. Journal of Cellular Biochemistry Suppl. (13 Part D): 297, 1989

Mapping the D.melanogaster En1A Enhancer Modules Responsible for Transcription Activation and Long-Distance Enhancer-Promoter Interactions. Acta Naturae 9(1): 99, 2017

CCAAT/enhancer binding protein binds and activates the promoter of the rat class I alcohol dehydrogenase gene. Archives of Biochemistry and Biophysics 285(2): 246-251, 1991

Ccaat enhancer binding protein c ebp binds and activates the promoter of the rat class i alcohol dehydrogenase adh gene. Hepatology 12(4 Part 2): 920, 1990

Tissue-specific in vivo protein-DNA interactions at the promoter region of the 63kd keratin gene during metamorphosis. Developmental Biology 198(1): 175, 1998

Tissue-specific in vivo protein-DNA interactions at the promoter region of the Xenopus 63 kDa keratin gene during metamorphosis. Nucleic Acids Research 23(21): 4502-4509, 1995