Optimization of ethanol production by Saccharomyces cerevisiae (ATCC 60868) and Pichia stipitis Y-7124: a response surface model for simultaneous hydrolysis and fermentation of wheat straw

Awafo, V.; Chahal, D.; Simpson, B.

Journal of food biochemistry 22(6): 489-509

1998


ISSN/ISBN: 0145-8884
DOI: 10.1111/j.1745-4514.1998.tb00258.x
Accession: 013167994

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Abstract
Optimization models are useful in ascertaining optimum parameters as they relate to a particular response. Ethanol production with different cellulase-systems and substrate concentrations were subjected to this model and the results revealed that crude unextracted cellulase-systems are more efficient than crude extracted cellulase-systems in producing hydrolysates for ethanol production with Pichia stipitis (Y-7124), Saccharomyces cerevisiae (AT 60868) and their mixture. Response surface plots showed that for 80-90% of high concentrations of delignified wheat straw up to 11% could be converted into ethanol by simultaneous hydrolysis with enzyme loading of 16 IU/mL and fermentation with P. stipitis. P. stipitis was found to be the most suitable yeast for the fermentation of mixed hexose and pentose sugars in the wheat straw hydrolysate.

Optimization of ethanol production by Saccharomyces cerevisiae (ATCC 60868) and Pichia stipitis Y-7124: a response surface model for simultaneous hydrolysis and fermentation of wheat straw