EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Reaction kinetic model of a proposed plasma membrane two-cycle H(+)-transport system of Chara corallina



Reaction kinetic model of a proposed plasma membrane two-cycle H(+)-transport system of Chara corallina



Proceedings of the National Academy of Sciences of the United States of America 89(8): 3261-3265



Biophysical and numerical analysis methods were used to characterize and model the transport protein that gives rise to the acid and alkaline regions of Chara. A measuring system that permits the detection of area-specific current-voltage curves was used. These current-voltage curves, obtained from the inward current regions of Chara, underwent a parallel shift when the alkaline region was inverted by means of an acid pH treatment. In this situation the reversal potential of this area shifted from -120 mV to -340 mV. Together with data obtained from experiments using a divided chamber system, these results suggest that a common transport protein generates inward and outward current regions of Chara. On the basis of these experimental findings, a reaction kinetic model is proposed that assigns two operational modes to the proposed transport protein. Switching between these modes generates either acid or alkaline behavior. Since the observed pH dependence of the postulated transporter is rather complex, a reaction kinetic saturation mechanism had to be incorporated into the model. This final 10-state reaction kinetic model provides an appropriate set of mathematical relations to fit the measured current-voltage curves by computer.

(PDF emailed within 0-6 h: $19.90)

Accession: 013181739

Download citation: RISBibTeXText

PMID: 1373492

DOI: 10.1073/pnas.89.8.3261



Related references

Reaction kinetic model of a proposed plasma membrane two cycle proton transport system of chara corallina. Proceedings of the National Academy of Sciences of the United States of America 89(8): 3261-3265, 1992

Mechanism of chloride ion transport at the plasma membrane of chara corallina 2. trans inhibition and the determination of hydrogen ion chloride ion binding order from a reaction kinetic model. Journal of Membrane Biology 58(2): 139-154, 1981

The mechanism of chloride transport at the plasma membrane of chara corallina 1. co transport with hydrogen ion. Journal of Membrane Biology 53(2): 129-142, 1980

Control of phosphate transport across the plasma membrane of Chara corallina. Journal of experimental botany 49(318): 13-19, 1998

Mechanism of Cl- transport at the plasma membrane of Chara corallina. II. Transinhibition and the determination of H+. Journal of membrane biology8(2): 139-153, 1981

Spatial and temporal control of ion transport at the plasma membrane of Chara corallina. Rensing, Ludger Cellular Clocks Series; Oscillations and morphogenesis: 413-425, 1993

Spatial organization of transport domains and subdomain formation in the plasma membrane of Chara corallina. Journal of Membrane Biology 147(3): 275-281, 1995

Induction of the Na+/Pi cotransport system in the plasma membrane of Chara corallina requires external Na+ and low levels of Pi. Plant, Cell and Environment 25(11): 1475-1481, 2002

The plasma membrane coat and its origin in chara corallina. Cram, W J Et Al (Ed ) Membrane Transport in Plants; Symposium, Prague, Czechoslovakia, Aug 15-21, 1983 592p Academia: Prague, Czechoslovakia; John Wiley And Sons: New York, N Y , Usa; Chichester, England Illus 274-276, 1984

Simultaneous Measurements of Cytoplasmic K Concentration and the Plasma Membrane Electrical Parameters in Single Membrane Samples of Chara corallina. Plant Physiology 82(2): 417-422, 1986

A Characeae Cells Plasma Membrane as a Model for Selection of Bioactive Compounds and Drugs: Interaction of HAMLET-Like Complexes with Ion Channels of Chara corallina Cells Plasmalemma. Journal of Membrane Biology 249(6): 801-811, 2016

Simultaneous measurements of cytoplasmic potassium concentration and the plasma membrane electrical parameters in single membrane samples of chara corallina. Plant Physiology (Rockville) 82(2): 417-422, 1986

The effect of pH on the OsO4--revealed structure of the plasma membrane of Chara corallina. Biochimica et biophysica acta: 330 (2) 141-146, 1973

Control of plasma membrane Cl- -fluxes in Chara corallina by external Cl- and light. Journal of experimental botany 31(120): 105-118, 1980