+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Reduced expression of alpha-tubulin genes in Arabidopsis thaliana specificity affects root growth and morphology, root hair development and root gravitropism



Reduced expression of alpha-tubulin genes in Arabidopsis thaliana specificity affects root growth and morphology, root hair development and root gravitropism



Plant journal: for cell and molecular biology 28(2): 145-157



Different alpha-tubulin cDNA sequences fused in an antisense orientation to a CaMV 35S promoter were introduced into Arabidopsis thaliana plants. Several independent transgenic lines that showed a moderate but clear reduction of alpha-tubulin gene expression (TUA6/AS lines) were obtained and phenotypically characterized. Although no apparent abnormalities were detected in the aerial parts of TUA6/AS plants, root development was severely affected. Cells in TUA6/AS root tips were found to contain aberrant microtubular structures, to expand abnormally and to be unable to undergo regular cell division. These cellular defects caused a dramatic radial expansion of the root tip and inhibited root elongation. In addition, TUA6/AS roots displayed ectopic formation of root hairs, root hair branching and a reduced ability to respond to gravitropic challenges. Our results contribute to an improved understanding of the different roles microtubules play during root development and demonstrate that reverse genetics is a powerful tool to analyze cytoskeletal functions during plant organogenesis.

(PDF emailed within 1 workday: $29.90)

Accession: 013182642

Download citation: RISBibTeXText


Related references

Reduced expression of alpha-tubulin genes in Arabidopsis thaliana specifically affects root growth and morphology, root hair development and root gravitropism. Plant Journal 28(2): 145-157, 2001

Root gravitropism and root hair development constitute coupled developmental responses regulated by auxin homeostasis in the Arabidopsis root apex. New Phytologist 197(4): 1130-1141, 2013

Root hair defective4 encodes a phosphatidylinositol-4-phosphate phosphatase required for proper root hair development in Arabidopsis thaliana. Plant Cell 20(2): 381-395, 2008

Myosin XIK of Arabidopsis thaliana accumulates at the root hair tip and is required for fast root hair growth. Plos One 8(10): E76745-E76745, 2014

Single-point ACT2 gene mutation in the Arabidopsis root hair mutant der1-3 affects overall actin organization, root growth and plant development. Annals of Botany, 2018

Involvement of Arabidopsis thaliana phospholipase D2 in root hydrotropism through the suppression of root gravitropism. Planta 231(2): 491-497, 2010

Involvement of Arabidopsis thaliana phospholipase Dzeta2 in root hydrotropism through the suppression of root gravitropism. Planta 231(2): 491-497, 2010

The axr4 auxin-resistant mutants of Arabidopsis thaliana define a gene important for root gravitropism and lateral root initiation. Plant Journal 7(2): 211-220, 1995

Analysis of the root-hair morphogenesis transcriptome reveals the molecular identity of six genes with roles in root-hair development in Arabidopsis. Plant Journal 45(1): 83-100, 2005

Growth in Turface® clay permits root hair phenotyping along the entire crown root in cereal crops and demonstrates that root hair growth can extend well beyond the root hair zone. Bmc Research Notes 8: 143-143, 2016

Knock-Down of Arabidopsis PLC5 Reduces Primary Root Growth and Secondary Root Formation While Overexpression Improves Drought Tolerance and Causes Stunted Root Hair Growth. Plant & Cell Physiology, 2018

S-nitrosoglutathione promotes cell wall remodelling, alters the transcriptional profile and induces root hair formation in the hairless root hair defective 6 (rhd6) mutant of Arabidopsis thaliana. New Phytologist (): -, 2016

ROOT HAIR DEFECTIVE SIX-LIKE4 (RSL4) promotes root hair elongation by transcriptionally regulating the expression of genes required for cell growth. New Phytologist 212(4): 944-953, 2016

Impact of copper oxide nanoparticles exposure on Arabidopsis thaliana growth, root system development, root lignificaion, and molecular level changes. Environmental Science and Pollution Research International 21(22): 12709-12722, 2015

Root growth, secondary root formation and root gravitropism in carotenoid-deficient seedlings of Zea mays L. Annals of Botany 55: 387-394, 1985