EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

RpoNo of the fish pathogen Vibrio (Listonella) anguillarum is essential for flagellum production and virulence by the water-borne but not intraperitoneal route of inoculation



RpoNo of the fish pathogen Vibrio (Listonella) anguillarum is essential for flagellum production and virulence by the water-borne but not intraperitoneal route of inoculation



Microbiology 143(12): 3849-3859



To investigate the involvement of RpoN in flagellum production and pathogenicity of Vibrio (Listonella) anguillarum, the rpoN gene was cloned and sequenced. The deduced product of the rpoN gene displayed strong homology to the alternative sigma factor (RpoN) of numerous species of bacteria. In addition, partial sequencing of rpoN-linked ORFs revealed a marked resemblance to similarly located ORFs in other bacterial species. A polar insertion or an in-frame deletion in the coding region of rpoN abolished expression of the flagellin subunits and resulted in loss of motility. Introduction of the rpoN gene of V. anguillarum or Pseudomonas putida into the rpoN mutants restored flagellation and motility. The rpoN mutants were proficient in the expression of other proposed virulence determinants of V. anguillarum, such as ability to grow under low available iron conditions, and expression of the LPS O-antigen and of haemolytic and proteolytic extracellular products. The infectivity of the rpoN mutants with respect to the wild-type strain was unaffected following intraperitoneal injection of fish but was reduced significantly when fish were immersed in bacteria-containing water. Thus, rpoN does not appear to regulate any factors required for virulence subsequent to penetration of the fish epithelium, but is important in the infection of fish by water-borne V. anguillarum.

(PDF emailed within 1 workday: $29.90)

Accession: 013187581

Download citation: RISBibTeXText



Related references

RpoN of the fish pathogen Vibrio (Listonella) anguillarum is essential for flagellum production and virulence by the water-borne but not intraperitoneal route of inoculation. Microbiology 143: 3849-3859, 1998

RpoN of the fish pathogen Vibrio (Listonella) anguillarum is essential for flagellum production and virulence by the water-borne but not intraperitoneal route of inoculation. Microbiology Reading 143(12): 3849-3859, 1997

Vibrio anguillarum as a stress-borne pathogen in cultured fresh water fish. Veterinary Medical Journal Giza 48(1): 1-6, 2000

Complete genome sequence of the marine fish pathogen Vibrio anguillarum harboring the pJM1 virulence plasmid and genomic comparison with other virulent strains of V. anguillarum and V. ordalii. Infection and Immunity 79(7): 2889-2900, 2011

Virulence mechanisms of the murine fish pathogen vibrio anguillarum. Abstracts of the Annual Meeting of the American Society for Microbiology 80: ABSTRACT 839, 1980

Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum. Infection and Immunity 18(2): 509-513, 1977

Genetic Determinants of Virulence in the Marine Fish Pathogen Vibrio anguillarum. Fish Pathology 46: 1-10, 2011

Evidence for plasmid contribution to the virulence of the fish pathogen vibrio anguillarum. Abstracts Of The Annual Meeting Of The American Society For Microbiology: 0, 1977

Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. Journal of Fish Diseases 34(9): 643-661, 2011

DNA sequencing of a plasmid with virulence from marine fish pathogen Vibrio anguillarum. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica 35(10): 956-959, 2003

The genetics of plasmid mediated virulence in the marine fish pathogen vibrio anguillarum. Crosa, J H (Ed ) Bacterial And Viral Diseases Of Fish: Molecular Studies X+86p A Washington Sea Grant Publication: Seattle, Washington Illus Paper : p21-30, 1983

Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum. Infection and Immunity 27(3): 897-902, 1980

Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775. Journal of Bacteriology 185(19): 5822-5830, 2003

Polypeptides p40, pOM2, are required for iron uptake and for virulence of the marine fish pathogen Vibrio anguillarum 775. Journal of Bacteriology 173(3): 1347-1352, 1991

A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system. Nature 284(5756): 566-568, 1980