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Structural insights into the mechanism of formation of cellulosomes probed by small angle X-ray scattering


Structural insights into the mechanism of formation of cellulosomes probed by small angle X-ray scattering



Journal of Biological Chemistry 279(53): 55985-55994



ISSN/ISBN: 0021-9258

PMID: 15502162

DOI: 10.1074/jbc.m408979200

Exploring the mechanism by which the multiprotein complexes of cellulolytic organisms, the cellulosomes, attain their exceptional synergy is a challenge for biologists. We have studied the solution structures of the Clostridium cellulolyticum cellulosomal enzyme Cel48F in the free and complexed states with cohesins from Clostridium thermocellum and Clostridium cellulolyticum by small angle x-ray scattering in order to investigate the conformational events likely to occur upon complexation. The solution structure of the free cellulase indicates that the dockerin module is folded, whereas the linker connecting the catalytic module to the dockerin is extended and flexible. Remarkably, the docking of the different cohesins onto Cel48F leads to a pleating of the linker. The global structure determined here allowed modeling of the atomic structure of the C. cellulolyticum dockerin-cohesin interface, highlighting the local differences between both organisms responsible for the species specificity.

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Accession: 013196504

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