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Effects of theophylline on the expression of RANTES in the airway smooth muscle cells of rats induced by TNF-alpha



Effects of theophylline on the expression of RANTES in the airway smooth muscle cells of rats induced by TNF-alpha



Wuhan Daxue Xuebao (Yixue Ban) 28(1): 77-80,88,89



Objective: To explore the roles of theophylline on the expression of RANTES and cAMP in the airway smooth muscle cells (ASMCs) of rats induced by TNF-alpha, and to investigate RANTES and theophylline in the pathogenesis of bronchial asthma. Methods: The trachea smooth muscle was obtained from Wistar rat, and ASMCs were dissected, purified, and cultured in medium. Then were treated with TNF-alpha (the final concentration was 10 mu g/L) and different concentration of theophylline and ELISA method was used to determine the concentration of RANTES. The level of cAMP was measured by radioimmunoassay. Results: After treated with both TNF-alpha and theophylline, the expression of RANTES in rat ASMCs was lower than in the group without theophylline, and the higher concentration of theophylline, the lower of expression of RANTES. Except the 5 mg/L group, a significant positive correlation existed between the theophylline groups and control group(P < 0.05). Comparing among different dose of theophylline group, the 5 mg/L group had no significant difference with 7.5 mg/L(P > 0.05). Conclusion: Theophylline, of which the concentration is above 7.5 mg/L, can inhibit the expression of RANTES in ASMCs of rats. And the inhibition of theophylline on RANTES was related with cAMP.

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