+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

In vitro formation of gerbera (Gerbera jamesonii Bolus) plantlets from capitulum explants

In vitro formation of gerbera (Gerbera jamesonii Bolus) plantlets from capitulum explants

Propagation of Ornamental Plants 7(1): 37-42

ISSN/ISBN: 1311-9109

The shoot regeneration capacity of gerbera capitulum explants at different ages was investigated. The effect of the exogenous cytokinin BA concentration on in vitro shoot regeneration on the capitulum explant was studied. Regenerated shoots were separated from the capitulum explants and cultured in nutrient media containing cytokinins BA or kinetin for multiplication. For in vitro rooting shoots were then transferred to rooting media with different concentrations of nutrient elements, IBA and IAA. In vitro rooted shoots were transferred to in vivo conditions and planted in cocopeat or in a mixture of cocopeat + perlite (1 : 1) for acclimatization. Immature capitula of 1.0-1.5 cm in diameter showed the best shoot regeneration capacity compared to those at medium age or fully mature ones. The gerbera cultivars tested showed different responses to cytokinin (BA) concentration with regard to shoot formation on the capitulum explants. The multiplication rate increased with increasing BA concentration in the medium. The medium containing BA at 2.5 or 5.0 mg l(-1) gave a higher multiplication rate than the medium with kinetin at 10.0 mg l(-1). All the rooting media tested gave 100% rooting. There were very slight differences between the different rooting media with respect to number of roots per shoot and root length. Results showed no significant difference in rooting between full or half strength Murashighe and Skoog (1962) medium. After transfer from the agar medium to in vivo conditions, plantlets showed a survival percentage of more than 90% in either cocopeat alone or cocopeat + perlite at the ratio of 1 : 1.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 013250272

Download citation: RISBibTeXText

Related references

Elimination of Cucumber mosaic virus from gerbera (Gerbera jamesonii) cv. Zingaro through in vitro chemotherapy of capitulum explants. Indian Journal of Biotechnology 16(4): 641-647, 2017

Efficient Tdz-induced regeneration from capitulum explants of Gerbera jamesonii Bolus ex Hooker F. - an ornamental plant with high aesthetic value. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2018

Ha ploids in gerbera jamesonii from in vitro cultured capitulum explants. Zeitschrift fuer Pflanzenzuechtung 79(2): 167-171, 1977

In vitro mass multiplication of gerbera (Gerbera jamesonii) using capitulum explant. Indian Journal of Agricultural Sciences 82(9): 768-774, 2012

Flowers capitulum disinfection for in vitro propagation of Gerbera jamesonii Bolus. Revista de la Facultad de Agronomia de la Universidad Del Zulia 31(4): 510-523, 2014

In vitro plantlet production from young capitulum explants of gerbera jamesonii. HortScience 20(1): 137-139, 1985

Gerbera plantlets from in vitro cultivated capitulum explants. Scientia Horticulturae 1(1): 117-119, 1973

Zur Differentialdiagnose des pibzlichen Welkeerreger-komplexes bei Gerbera jamesonii Bolus - Diagnose différentielle des champignons phytopathogènes sur G.j - On the differential diagnosis of fungal wilt pathogens in Gerbera jamesonii Bolus. Nachrichtenbl. Pflanzenschutz Ddr, Berlin 39(1): 14-15, 1985

Commercial multiplication of gerbera (Gerbera jamesonii Bolus) through in vitro shoot tip culture. Journal of Ornamental Horticulture New Series 6(4): 303-309, 2003

In vitro culture of gerbera (Gerbera jamesonii H. Bolus) and its acclimation in greenhouse. Bioagro 12(3): 75-80, 2000

Callusing and regeneration response of in vitro derived leaf explants of gerbera (Gerbera jamesonii). Indian Journal of Agricultural Sciences 88(6): 860-864, 2018

Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers. Plant Cell, Tissue and Organ Culture 104(1): 131-135, 2011

In vitro clonal propagation of gerbera (Gerbera jamesonii Bolus). Buletin Penelitian Hortikultura 17(4): 34-43, 1989

In vitro studies on somatic embryogonesis and shoot proliferation in gerbera (Gerbera jamesonii Bolus ex Hooker F.) cv. Alsmeera. Journal of Ornamental Horticulture New Series 8(3): 196-200, 2005

Rapid in vitro regeneration of Gerbera jamesonii (H. Bolus ex Hook. f.) from different explants. Indian Journal of Biotechnology 3(4): 584-588, 2004